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HFL-I
Cat.No.: CSC-C9189W
Species: Human
Source: Lung
Morphology: fibroblast-like
Cell Type: Fibroblast
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HFL-I, also known as HFL-1, is a normal human fibroblast cell line isolated from human fetal lung tissue. These cells are a primary component of loose connective tissue and originate from mesenchymal cells during the embryonic stage. Similar to other fibroblasts, HFL-I cells exhibit an elongated, spindle-shaped morphology, possess abundant cytoplasm, and have prominent fibrous protrusions.
Embryonic lung fibroblasts play a crucial role in embryonic development, tissue repair, and disease research. For example, during development, these cells secrete various growth factors and cytokines, such as transforming growth factor-β (TGF-β), fibroblast growth factor (FGF), and epidermal growth factor (EGF), which are involved in alveolar formation, differentiation, and maturation. Additionally, they produce and maintain the extracellular matrix (ECM) within lung tissue, including collagen and elastin, which not only provide structural support to the lungs but also participate in the expansion and contraction of alveoli. In terms of tissue repair, embryonic lung fibroblasts are vital in repairing cellular degeneration, necrosis, and tissue defects of varying degrees. In the treatment of lung diseases such as pulmonary fibrosis and lung cancer, these cells can differentiate into specific cell types, promoting lung tissue repair and regeneration. In disease research, HFL-I is frequently used to investigate the mechanisms underlying pulmonary fibrosis, asthma, and chronic obstructive pulmonary disease (COPD). In-depth studies of the biological characteristics and regulatory mechanisms of embryonic lung fibroblasts are essential for developing novel therapeutic strategies.
Fig. 1. Micrograph of HFL-I (Okano, N., 2017).
MRJP Prolonged the Life Span and Promoted the Proliferation of HFL-I Cells
Royal jelly (RJ), secreted from honeybees, has multiple known bioactivities, such as antioxidant and immunoregulatory effects. However, its specific impact on human cell aging required further exploration. Jiang's team investigated the anti-senescence activity of major royal jelly proteins (MRJPs) on human embryonic lung fibroblast (HFL-I) cells. Results indicated that MRJPs notably enhanced cell proliferation, reduced senescence, and prolonged telomeres, suggesting MRJPs' potential as anti-senescence agents in human cells.
Cellular morphological observations revealed that MRJPs affected the appearance of HFL-I cells. In medium A (Fig. 1a), most cells appeared flat with unclear boundaries, characteristic of senescence. Cells were often unattached and lost their spindle shape. Conversely, cells in medium C (Fig. 1c) and medium D (Fig. 1d) maintained a healthy shape and size with distinct boundaries. Cells in medium B (Fig. 1b) and medium E (Fig. 1e) showed some signs of senescence. Compared to medium A, PDL increased by 12.4%, 31.2%, 24.0%, and 10.4% in media B, C, D, and E, respectively. Medium C cells had the highest PDL, suggesting 0.2 mg/ml MRJPs (medium C) as the optimal dose for extending HFL-I cell lifespan (Fig. 2a). By the fourth day, the cells' proliferation activity (absorbance) in media A, B, C, D, and E was 0.39, 0.40, 0.45, 0.44, and 0.42, respectively (Fig. 2b). Compared to medium A, the relative proliferation activity (RPA) in media B, C, D, and E increased by 1.0%, 11.9%, 11.1%, and 7.7%.
Fig. 1. Cellular morphology of HFL-I cells supplied with different concentrations of MRJPs or BSA (Jiang CM, Liu X, et al., 2018).
Fig. 2. (a) Lifespans of HFL-I cells supplied with different concentrations of MRJPs or BSA. (b) Proliferation effect of MRJPs after 4 d (Jiang CM, Liu X, et al., 2018).
In Vitro Reaction of Cells Derived from Human Normal Lung Tissues to Carbon-Ion Beam Irradiation
The translation of X-ray therapy data to CIRT is hindered by the unclear relative biological effectiveness (RBE) of carbon-ion beams in normal lung tissues. To overcome this, Okano utilized immortalized human small airway epithelial cells (iSAECs), human lung fibroblasts (HFL-1), and A549 lung cancer cells to compare the effects of X-ray and carbon-ion beam irradiation. He employed crystal violet staining (CVS) and clonogenic assays to generate survival curves, demonstrating the CVS assay's efficacy as an alternative for assessing the RBE of carbon-ion beams in non-clonogenic cells.
Using the CVS assay, irradiation of iSAECs and HFL-I cells with X-rays or carbon-ion beams decreased absorbance in a dose-dependent manner at low doses (Fig. 3A and B). However, high-dose irradiation caused cells to lose proliferative ability and show enlarged morphology (Fig. 3C and D), characteristic of senescence. Microscopic observation confirmed fewer cells after high-dose exposure than estimated by CVS assay, indicating an overestimation of the surviving fraction (Fig. 4). The induction of senescence in these cells was verified by increased SA-β-gal positivity 10 days post-irradiation with high doses. These findings indicate that the high staining intensity of the samples could be attributed to senescent cells with enlarged bodies that accumulated on the culture plates in response to high-dose irradiation, leading to the overestimation of the surviving fraction in the CVS assay.
Fig. 3. (A-B) Sensitivity of iSAECs and HFL-I cells to X-ray and carbon-ion beam irradiation assessed by using the CVS assay. (C-D) Representative micrographs of iSAECs and HFL-I cells exposed to X-ray and carbon-ion beam irradiation (Okano, N., 2017).
Fig. 4. Induction of senescence by X-ray and carbon-ion beam irradiation of iSAECs and HFL-I cells (Okano, N., 2017).
HFL-I is a fibroblast cell line that was isolated from the lung of a White, normal embryo. This cell line can be used in toxicology research.
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Average Rating: 5.0 | 5 Scientist has reviewed this product
High-quality & reliable
The cells performed very well in my experiments, a testament to their quality and reliability.
18 Mar 2023
Ease of use
After sales services
Value for money
High-quality & reliable
The cells performed very well in my experiments, a testament to their quality and reliability.
18 Mar 2023
Ease of use
After sales services
Value for money
High-quality & reliable
The cells performed very well in my experiments, a testament to their quality and reliability.
18 Mar 2023
Ease of use
After sales services
Value for money
High-quality & reliable
The cells performed very well in my experiments, a testament to their quality and reliability.
18 Mar 2023
Ease of use
After sales services
Value for money
High-quality & reliable
The cells performed very well in my experiments, a testament to their quality and reliability.
18 Mar 2023
Ease of use
After sales services
Value for money
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