Carrageenan-Induced Air Pouch Synovitis Model

Creative Bioarray offers a carrageenan-induced air pouch synovitis model, designed to facilitate preclinical research for the screening of anti-inflammatory drugs and to conduct in vivo efficacy tests. This model is an essential tool for researchers aiming to evaluate the potential of drug candidates in reducing inflammation, providing a reliable platform for evaluating the efficacy and safety of drugs before they advance to clinical trials. Our services are tailored to meet the specific needs of each study, ensuring accurate and reproducible results that can significantly contribute to the drug development process.

The subcutaneous air pouch is a model used to study acute inflammation in vivo. It was first described by Selye in 1953. The pouch is created by injecting sterile air into the intra-scapular area of the rat or mouse's back. Later, various irritants can be injected into the pouch to induce inflammation. The lining of the pouch is composed primarily of macrophage-like (Type A) and fibroblast-like (Type B) cells, similar to the synovial cavity. Injection of a carrageenan solution into the pouch triggers an inflammatory reaction characterized by cell infiltration, increased exudate, and the production of pro-inflammatory mediators, including prostaglandins, leukotrienes, cytokines, and oxidative stress response components.

Our Carrageenan-Induced Air Pouch Synovitis Model

• Available Animal

• Rat
• Mouse

• Modeling Method

After acclimation, sterile air is injected into the subcutaneous tissue at the midline of their backs. Following this, a solution of carrageenan in sterile saline is injected into the animals' air pouch.

• Endpoints

• Histology analysis: H&E staining
• Clinical observation
• Cytokine analysis
• Lavage fluid analysis: cell count, MDA, SOD, etc.
• Other customized endpoints

Example Data

Fig. 1 Subcutaneous administration of WAS attenuates pro-inflammatory cytokine production and immune cell recruitment in a carrageenan-induced mouse model of acute inflammation. (Ahn et al. 2021)

Quotation and Ordering

At Creative Bioarray, we are dedicated to delivering our clients with the most cost-effective pre-clinical in vivo drug efficacy evaluation services, ensuring that our solutions meet the highest standards of quality and efficiency. If you are interested in our services, please feel free to contact us at any time or submit an inquiry to us directly.

References

1. Duarte, D.B., et al. Models of inflammation: carrageenan air pouch. Current protocols in pharmacology, 2012, 56(1): 5.6. 1-5.6. 8.
2. Ahn, J.H., et al. Water extract of Artemisia scoparia Waldst. & Kitam suppresses LPS-induced cytokine production and NLRP3 inflammasome activation in macrophages and alleviates carrageenan-induced acute inflammation in mice. Journal of Ethnopharmacology, 2021, 268: 113606.

For research use only. Not for any other purpose.