Spared Nerve Injury (SNI) Model
Creative Bioarray offers the spared nerve injury (SNI) model, a widely recognized experimental model, to our esteemed clients engaged in the study of neuropathic pain mechanisms or the preclinical evaluation of analgesic drug efficacy. Our team of experienced professionals ensures that all procedures are conducted with the highest standards of precision and ethical considerations, providing our clients with reliable and reproducible data to support your research. Whether you are aiming to uncover new insights into the molecular and cellular pathways involved in neuropathic pain or seeking to validate the effectiveness of your analgesic compounds, Creative Bioarray's SNI model is your trusted partner in advancing the frontiers of pain research.
Peripheral neuropathic pain is produced by multiple etiological factors that initiate a number of diverse mechanisms operating at different sites and at different times and expressed both within, and across different disease states. Unraveling the mechanisms involved requires laboratory animal models that replicate as far as possible, the different pathophysiological changes present in patients. The SNI model is a common experimental technique used in neuroscience research to induce neuropathic pain in animals, typically rodents. Researchers use the SNI model to study the mechanisms underlying neuropathic pain and to test potential treatments for chronic pain conditions.
Our Spared Nerve Injury (SNI) Model
- Available Animal
Rat
- Modeling Method
In the model group, after anesthetization, an approximately 2 cm incision is made to the upper edge of the left hind limb. Afterward, the muscle is separated gently, and the main trunk of the sciatic nerve and its 3 branches are exposed and bluntly separated. Then, the common peroneal nerve and tibial nerve are ligated with 4-0 silk sutures. Both nerves are severed at about 2 cm after ligation and the sural nerve was preserved. In the sham group, the sciatic nerve and its 3 branches are only exposed.
Fig. 1 The procedure of Spared Nerve Injury (SNI) Model. (Duraku et al. 2012)
- Endpoints
- Body weight
- Behavioral tests: Von Frey test, Hot plate test, etc.
- qPCR or Western blot
- Histology analysis
- Other customized endpoints
Example Data
Fig. 2 Inhibition of P2X7R in the amygdala elevated the nociceptive threshold of MWT and TWD. (A) MWT was measured in rats of different groups with von Frey filaments. (B) TWD was measured in rats of different groups with von Frey filaments. (C) TST was performed in rats of different groups. (D) FST was carried out in rats of different groups. (E) OFT was conducted in rats of different groups. (F) SPT was performed in rats of different groups (21 days). (Hu et al. 2020)
Moreover, we also provide other neuropathic pain models that maybe you are interested in:
- Diabetes-Induced Neuropathic Pain Model
- Chemotherapy-Induced Neuropathic Pain Model
- Chronic Constriction Injury (CCI) Model
- Partial Sciatic Nerve Ligation (PSL/PSNL) Model
- Spinal Nerve Ligation (SNL) Model
Quotation and Ordering
Creative Bioarray is dedicated to providing stable disease models for our clients to accelerate your drug development. If you are interested in our services, please feel free to contact us at any time or submit an inquiry to us directly.
References
- Hu, X., et al. Inhibition of P2X7R in the amygdala ameliorates symptoms of neuropathic pain after spared nerve injury in rats. Brain, Behavior, and Immunity, 2020, 88: 507-514.
- Decosterd, I., Woolf, C.J. Spared nerve injury: an animal model of persistent peripheral neuropathic pain. Pain, 2000, 87(2): 149-158.
- Duraku, L.S., et al. Spatiotemporal dynamics of re-innervation and hyperinnervation patterns by uninjured CGRP fibers in the rat foot sole epidermis after nerve injury. Mol Pain. 2012; 8:61.
