Anti-CD40 Antibody Induced Colitis Model

Creative Bioarray stands at the forefront of preclinical research, providing cutting-edge models the Anti-CD40 Antibody Induced Colitis Model to propel your drug discovery initiatives forward. Partner with us to unlock the potential of your drug candidates and advance the fight against inflammatory bowel disease (IBD).

Critical Role of CD40 in Intestinal Inflammation

The interaction between CD40 and its ligand CD154 (CD40L) is crucial for the initiation and maintenance of T cell-mediated intestinal inflammation, a hallmark of IBD. This interaction is evident in both human IBD patients and mouse models of IBD, where CD40+ antigen-presenting cells (APCs) are found in close association with CD40L+ T cells within the inflamed intestinal tissue. By activating CD40 with an agonist antibody, the model effectively replicates this critical interaction, providing a unique platform to study the inflammatory pathways and evaluate potential therapeutic interventions.

The result of CD40 activation in this model is a robust innate immune response characterized by the upregulation of IL-23 and IL-12 mRNA in myeloid cells, increased levels of proinflammatory cytokines in the serum, and the development of colonic inflammation. This response closely mirrors the inflammatory environment seen in IBD, making the Anti-CD40 Induced Colitis Model an invaluable tool for understanding the disease's pathogenesis and for testing new therapeutic strategies aimed at mitigating intestinal inflammation.

Our Anti-CD40 Antibody Induced Colitis Model

Animal Species

• Mouse

Modeling Method

• SCID mice or RAG2^-/- mice are injected (i.p.) with anti-CD40 monoclonal antibodies to induce ulcerative colitis.

Fig. 1 Modeling method of Anti-CD40 Induced Colitis Model

Endpoints

• Body weight
• DAI score
• Colon weight
• Colon length
• Cytokine analysis
• qPCR or Western blot
• Histology analysis
• Other customized endpoints according to your specific needs

Example Data

Fig. 2 Anti-CD40 Stimulation Induces Wasting Disease and Intestinal Inflammation. Rag1 KO mice received 200 mg CD40 mAb i.p. Control mice received isotype control antibodies or PBS. (A) Weight as a percentage of the initial weight at day 0. (B) H&E staining of proximal and intermediate colon. Epithelial hyperplasia (H), epithelial cell damage (ED), leucocytic cell clusters (LC) in the lamina propria, and a depletion of goblet cells (GCD) were found after CD40 stimulation. (C) Colitis score (Uhlig et al. 2006)

Quotation and Ordering

By leveraging our state-of-the-art facilities and deep scientific expertise, Creative Bioarray offers you a robust solution that not only accelerates your drug discovery process but also ensures the rigorous evaluation and optimization of your compounds for superior efficacy and safety. If you are interested in our services, please feel free to contact us at any time or submit an inquiry to us directly.

Reference

1. Uhlig, H.H., et al. Differential activity of IL-12 and IL-23 in mucosal and systemic innate immune pathology. Immunity, 2006, 25(2): 309-318.

For research use only. Not for any other purpose.