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Services
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Cell Services
- Cell Line Authentication
- Cell Surface Marker Validation Service
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Cell Line Testing and Assays
- Toxicology Assay
- Drug-Resistant Cell Models
- Cell Viability Assays
- Cell Proliferation Assays
- Cell Migration Assays
- Soft Agar Colony Formation Assay Service
- SRB Assay
- Cell Apoptosis Assays
- Cell Cycle Assays
- Cell Angiogenesis Assays
- DNA/RNA Extraction
- Custom Cell & Tissue Lysate Service
- Cellular Phosphorylation Assays
- Stability Testing
- Sterility Testing
- Endotoxin Detection and Removal
- Phagocytosis Assays
- Cell-Based Screening and Profiling Services
- 3D-Based Services
- Custom Cell Services
- Cell-based LNP Evaluation
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Stem Cell Research
- iPSC Generation
- iPSC Characterization
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iPSC Differentiation
- Neural Stem Cells Differentiation Service from iPSC
- Astrocyte Differentiation Service from iPSC
- Retinal Pigment Epithelium (RPE) Differentiation Service from iPSC
- Cardiomyocyte Differentiation Service from iPSC
- T Cell, NK Cell Differentiation Service from iPSC
- Hepatocyte Differentiation Service from iPSC
- Beta Cell Differentiation Service from iPSC
- Brain Organoid Differentiation Service from iPSC
- Cardiac Organoid Differentiation Service from iPSC
- Kidney Organoid Differentiation Service from iPSC
- GABAnergic Neuron Differentiation Service from iPSC
- Undifferentiated iPSC Detection
- iPSC Gene Editing
- iPSC Expanding Service
- MSC Services
- Stem Cell Assay Development and Screening
- Cell Immortalization
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ISH/FISH Services
- In Situ Hybridization (ISH) & RNAscope Service
- Fluorescent In Situ Hybridization
- FISH Probe Design, Synthesis and Testing Service
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FISH Applications
- Multicolor FISH (M-FISH) Analysis
- Chromosome Analysis of ES and iPS Cells
- RNA FISH in Plant Service
- Mouse Model and PDX Analysis (FISH)
- Cell Transplantation Analysis (FISH)
- In Situ Detection of CAR-T Cells & Oncolytic Viruses
- CAR-T/CAR-NK Target Assessment Service (ISH)
- ImmunoFISH Analysis (FISH+IHC)
- Splice Variant Analysis (FISH)
- Telomere Length Analysis (Q-FISH)
- Telomere Length Analysis (qPCR assay)
- FISH Analysis of Microorganisms
- Neoplasms FISH Analysis
- CARD-FISH for Environmental Microorganisms (FISH)
- FISH Quality Control Services
- QuantiGene Plex Assay
- Circulating Tumor Cell (CTC) FISH
- mtRNA Analysis (FISH)
- In Situ Detection of Chemokines/Cytokines
- In Situ Detection of Virus
- Transgene Mapping (FISH)
- Transgene Mapping (Locus Amplification & Sequencing)
- Stable Cell Line Genetic Stability Testing
- Genetic Stability Testing (Locus Amplification & Sequencing + ddPCR)
- Clonality Analysis Service (FISH)
- Karyotyping (G-banded) Service
- Animal Chromosome Analysis (G-banded) Service
- I-FISH Service
- AAV Biodistribution Analysis (RNA ISH)
- Molecular Karyotyping (aCGH)
- Droplet Digital PCR (ddPCR) Service
- Digital ISH Image Quantification and Statistical Analysis
- SCE (Sister Chromatid Exchange) Analysis
- Biosample Services
- Histology Services
- Exosome Research Services
- In Vitro DMPK Services
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In Vivo DMPK Services
- Pharmacokinetic and Toxicokinetic
- PK/PD Biomarker Analysis
- Bioavailability and Bioequivalence
- Bioanalytical Package
- Metabolite Profiling and Identification
- In Vivo Toxicity Study
- Mass Balance, Excretion and Expired Air Collection
- Administration Routes and Biofluid Sampling
- Quantitative Tissue Distribution
- Target Tissue Exposure
- In Vivo Blood-Brain-Barrier Assay
- Drug Toxicity Services
I-FISH Service
High-Precision Interphase Fluorescence In Situ Hybridization for Accurate Copy Number and Localization Analysis
What is I-FISH?
Interphase Fluorescence In Situ Hybridization (I-FISH) is a powerful cytogenetic technique used to detect and localize specific DNA sequences within interphase nuclei. It provides unparalleled accuracy in determining copy number variations (CNVs) and chromosomal localization of genetic elements.
Key Features of I-FISH
- High-resolution detection of DNA sequences in interphase nuclei.
- Precise quantification of copy number variations (CNVs).
- Direct visualization of chromosomal integration sites.
- Suitable for complex genetic studies and lentiviral vector analysis.
Figure 1. Determination of TNNT2 copy number in TNNT2/ACTC reporter-modified iPSCs. Representative images of I-FISH signals with complementary probe generated from TNNT2 vector in the cells at passages 34, 37, 40, 43 and 46. As a negative control, reporter unmodified cells were used. Images were taken at 100x magnification.
Applications of I-FISH
I-FISH is widely used in both research and clinical settings for a variety of applications:
Gene Copy Number Analysis
Accurately determine the copy number of specific genes or genetic elements, such as TNNT2 and ACTC, in cell populations.
Chromosomal Integration Studies
Visualize the chromosomal localization and distribution of lentiviral vectors or other genetic inserts.
Cancer and Genetic Research
Analyze chromosomal abnormalities translocations, and amplifications in cancer and other genetic disorders.
Pluripotent Stem Cell Studies
Monitor the stability of reporter sequences in ipsCs during prolonged in vitro culture.
Lentiviral Vector Analysis
Determine the integration patterns and stability of lentiviral vectors in host genomes.
High-Throughput Screening (HTS)
Use l-FlSH to validate reporter-modified cell lines for HTS applications.
Our I-FISH Service Workflow
We provide a streamlined and reliable I-FISH service to meet your research needs:
Sample Preparation
Fixation and preparation of your cell ortissue samples for I-FISH analysis.
Probe Hybridization
Hybridization of biotin-labeled probesspecific to your target sequences.
Signal Detection
Visualization and quantification ofhybridization signals using fluorescence microscopy.
Quotation and ordering
Our customer service representatives are available 24hr a day! We thank you for choosing Creative Bioarray at your preferred I-FISH Service.
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For research use only. Not for any other purpose.
