Droplet Digital PCR (ddPCR) Service

Droplet Digital PCR (ddPCR) is a great technology that provides ultrasensitive nucleic acid detection and absolute quantification.

The ddPCR method involves dividing nucleic acid samples into numerous microdroplets of known volume within a water-oil emulsion. Amplification of specific nucleic acid sequences occurs individually within each microdroplet using the same process and materials typically employed in a standard TaqMan® probe-based assay. Following amplification, fluorescence detection is used to categorize each droplet as either PCR-positive or PCR-negative. The concentration of target DNA/RNA templates (copies/µl) in the initial sample is then determined using a Poisson distribution based on the counted droplets.

Whether you are interested in pinpointing particular allelic variations in genetic studies, detecting structural alterations in genomes for medical diagnosis, or even identifying trace amounts of pathogens in environmental studies, the unique capability of ddPCR to provide absolute quantification distinguishes it from alternative methods. Absolute quantification is essential for tasks demanding a precise and direct measurement of target molecules, providing a distinct edge over relative quantification approaches. Our proficient team at Creative Bioarray possesses the expertise and technical proficiency to support your ddPCR analysis.

ddPCR can be performed as a standalone analysis or combined with our Transgene Mapping (Locus Amplification & Sequencing) assays, which provide comprehensive information about the transgene vector, including the vector copy numbers, vector integration sites, and vector integrity.

ddPCR can also be used to do Pre-MCB Clone Check Clone Characterization. When scaling up production, cells sometimes show a diminished growth rate. This can be the result of a high vector copy number. Excessive copies can create a metabolic burden on the cells resulting in stress, imbalanced gene expression, and viability issues.

Figure 1. qPCR vs Droplet Digital PCR (ddPCR). With qPCR, one measurement is made whereas with Droplet Digital PCR (ddPCR), many measurements are made.

Advantages of Droplet Digital PCR (ddPCR) Service

Direct absolute quantification
Higher sensitivity
Reduced susceptibility to PCR inhibitors
Higher tolerance to sample impurities
Ability to multiplex and analyze multiple targets in a single reaction

Droplet Digital PCR (ddPCR) Service Applications

Here at Creative Bioarray, we offer a comprehensive portfolio of ddPCR assays with wide-ranging research and diagnostic applications, including:

Absolute quantification of nucleic acid targets
Detection of rare mutations or variants
Copy number variation analysis
Gene expression analysis
NGS pre- and post-run validation
Gene editing event detection
Gene expression and miRNA analysis
Biodistribution: CAR-T and Gene therapy vectors

Compatible sample types of Droplet Digital PCR (ddPCR) Service:

Tissue
Fresh
Frozen
FFPE
Cells, exosomes
Liquid biopsies: Blood, BM, CSF, Saliva, Tears, Urine, cfDNA, cfRNA

Quotation and ordering

Our customer service representatives are available 24hr a day! We thank you for choosing Creative Bioarray at your preferred Droplet Digital PCR (ddPCR) Service.

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