Retinal Pigment Epithelium (RPE) Differentiation Service from iPSC
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There are multiple functions of the retinal pigment epithelium (RPE) that contribute to the vision process: it absorbs light, moves nutrients and ions, participates in the retinoid cycle, phagocytoses outer layers of photoreceptors, and secretes growth factors. Destruction of the retina compromises the integrity of the RPE and eventually causes blurred vision and even more serious ocular diseases. Compared to tissue-isolated retinal pigment epithelial cells, RPE cells derived from iPSC provide a continuous supply of RPE cells with identical backgrounds.
Creative Bioarray offers professional RPE cell differentiation service from iPSC. We combine state-of-the-art cell culture equipment and quality control measures to ensure that the RPE cells we produce are extremely pure, highly viable, and maintain stable physiological functions to support your research.
Applications and advantages of iPSC-RPE cell lines:
- iPSC can be obtained from adult cells without relying on embryonic tissues, avoiding ethical issues.
- They can be used in the research of many retinal diseases, such as age-related macular degeneration and retinitis pigmentosa, aiding basic research and drug screening
- iPSC-RPE cells can be used for cell replacement therapy for vision restoration and show therapeutic potential in animal models.
Our RPE Differentiation Service Includes
- iPSC preparation:
We accept client-provided iPSCs.
We also offer reprogramming services from somatic cells to iPSCs using client-provided somatic cell samples (e.g., skin cells, blood cells).
- RPE differentiation:
Employ automated equipment management and strictly controlled differentiation protocols for efficient and rapid induction of iPSCs into RPE cells.
- Cell identification and validation:
Ensure that differentiated RPE cells exhibit normal morphology, characteristics, and function, confirmed through assessments such as:
Morphological analysis: Typical cobblestone morphology
Biomarker testing: Expression of RPE-specific markers Bestrophin 1 (BEST1)/RPE65
Functional tests: Phagocytosis and barrier function assessments
- Cell culture and preservation:
Culture the differentiated RPE cells in vitro and provide long-term storage solutions for future research or other applications.
Delivery
- Cryopreserved iPSC-derived RPE: 60-80% BEST1+/RPE65+ cells
- Recovery Test, Mycoplasma Test
- Detailed cell identification and analysis report
- Protocols for passaging and technical support
- Additional markers (available upon request)
Our Service Highlights
- Highly efficient differentiation: We utilize cutting-edge differentiation technologies combined with an optimized culture environment to ensure efficient differentiation of RPE cells and high-quality cell production.
- Highly customized: We provide customized iPSC preparation and differentiation solutions to meet the specific needs of our customers.
- Strict quality control: From iPSC preparation to RPE cell differentiation, we implement strict testing and validation processes for each step.
- Long-term support: We provide comprehensive after-sales service and technical support to help customers solve any problems in the process of use, and provide regular technical updates and optimization suggestions.
FAQ
1. How do iPSC-derived RPE cells differ from native RPE cells?
iPSC-RPE cells are induced from iPSC after a rigorous differentiation and quality control process. It is genetically consistent with the donor and has similar physiological functions and morphological characteristics to the native RPE cells. The difference is that iPSC-RPE can be customized to the specific conditions of the patient and can be mass-produced in vitro. Moreover, due to the complexity of iPSC reprogramming and differentiation, some of its properties may differ from those of native cells.
2. What can these differentiated RPE cells be used for?
Our dedicated RPE cells can be used to investigate retinal development and disease models, for example studying the pathogenesis of retinal pathologies such as age-related macular degeneration (AMD). They can also serve as eye-models for toxicology studies, research and drug discovery.
3. What do I need to provide if I want to use your iPSC - RPE differentiation service?
If you wish to use our service, you can provide your own reliably sourced induced pluripotent stem cells (iPSC). Also, you have to let us know what exactly you need from the cells — number of cells, purity, and purpose. We can also provide suitable iPSC sourcing recommendations and assist you in obtaining the appropriate iPSCs according to your needs.
Quotation and Ordering
If you have any special need in retinal pigment epithelium differentiation service, do not hesitate to contact us. Please let us know what you need and we will accommodate you. We look forward to working with you.
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