T Cell Immortalization Service
Antigen-specific T cells play an important role in cellular immune response against cancer. The ability to isolate, maintain, and characterize tumor-specific T cells is a prerequisite to studying anticancer immune response and developing novel strategies for cancer immunotherapy. And T cells can also be used to screen potential drug candidates for their effects on T cell function and viability.
However, the life span of human T cells in vitro is usually very short and is limited by the onset of cellular senescence. To establish long-term, antigen-specific T-cells lines and clones, Creative Bioarray offers T cell immortalization service for you. In 2023, we established Immortalized Human T Cells using Epigenetic Approach successfully. Immortalized human T cells from Creative Bioarray can be cultured continuously for 20 passages. In addition, we also have other T cell immortalization methods available for you.
Methods for Establishing Immortalized T Cells
- Epigenetic Induction of Cell Growth
The Epigenetic Induction of Cells Growth (No viral gene) is a great method to establish the immortalized cell lines. Immortalized Human T Cells were successfully developed utilizing the epigenetic approach. The immortalized human T cells from Creative Bioarray can be consistently cultured for up to 20 passages. We are confident in using this method for T cell immortalization.
Figure 1. Human T cell immortalization by Epigenetic Induction of Cell Growth. Growing T cells at Day 2 after stimulation. NOTE: Non-fused control T cells can be cultured in the same conditions for 2 weeks before showing deteriorating cell growth.
Figure 2. Human T cell immortalization by Epigenetic Induction of Cell Growth. Growing T cells at Week 3 after stimulation.
- Overexpression of hTERT Gene
Some published literature indicates that hTERT gene transfection can immortalize human T cells. PBMCs were stimulated with antigens, and then infected with a murine leukemia virus-based retroviral vector carrying an immortalizing gene, the human telomerase-reverse transcriptase (hTERT) gene. Since such vectors can only integrate in dividing cells, only antigen-activated T cells are efficiently transduced. Using this approach, the immortalized T cell lines that maintained strictly IL-2-dependent growth and MHC-restricted, antigen-specific responsiveness, some of which have been in continuous culture for longer than 1 year, far in excess of the survival of parallel control no immortalized cultures. If you are interested in using this method, Creative Bioarray would be willing to try it for you.
- Overexpression of Oncogenes
Some scientists have established Immortalize human T cells using overexpression of c-Ha-ras and c-myc oncogenes. The cell lines kept growing for 3 months after establishment without a decrease in growth rate. The cells did not require interleukin-2(IL-2) for their growth, but addition of IL-2 stimulated the growth of these cells. Flow cytometric analysis revealed that these cells were T cells expressing CD4 or CD8 antigens. If you are interested in employing this approach, Creative Bioarray would be willing to provide the service for you.
Applications of Immortalized T Cells
- Studying T cell biology and function: Immortalized T cell lines can be used to investigate various aspects of T cell biology, such as activation, differentiation, and signaling pathways.
- Drug screening and development: Immortalized T cell lines can be used to screen potential drug candidates for their effects on T cell function and viability.
- Vaccine development: Immortalized T cell lines can be used to study immune responses to vaccines and to evaluate vaccine efficacy.
- Immunotherapy research: Immortalized T cell lines can be used to investigate novel immunotherapies, such as chimeric antigen receptor (CAR) T cell therapy.
- Infectious disease research: Immortalized T cell lines can be infected with pathogens to study host-pathogen interactions and immune responses to infection.
Creative Bioarray's T Cell Immortalization Service has the following Features:
- Immortalized cell lines from any species
- Different methods of cell immortalization you can choose
- Characterization according to your requirements
- Projects are designed and handled by experienced scientists.
- Short turn-around time
- Competitive pricing
Quotation and ordering
Our customer service representatives are available 24hr a day! We thank you for considering Creative Bioarray as your T Cell Immortalization Service partner.
Explore Other Options
