Cell Migration and Invasion Assay Services

Migration is a key property of live cells and critical for normal development, immune response, and disease processes such as cancer metastasis and inflammation. Methods to examine cell migration are very useful and important for a wide range of biomedical research such as cancer biology, immunology, vascular biology, cell biology and developmental biology. With comprehensive list of cell lines in our inventory, Creative Bioarray provides cell migration and Invasion Assay Services to all of our customers.

Creative Bioarray Cell Migration and Invasion Assay advantages

• Assays are performed using various types of cells: Cell lines, Primary human or murine keratinocytes, Primary human or murine fibroblasts.
• Quantitative, Real time detection
• High sensitivity
• Choose suitable cell migration assay format according to the customer’s requirements.

Technique and procedures

Two main types of methods are available at Creative Bioarray:

Cell culture wound-healing assays

The wound-healing assay is simple, inexpensive method to study directional cell migration in vitro. This method mimics cell migration during wound healing in vivo. A “wound” is generated on a confluent cell monolayer. The speed of wound closure and cell migration can be quantified by taking snapshot pictures with a regular inverted microscope at several time intervals. More detailed cell migratory behavior can be documented using the time-lapse microscopy system.

Suitable Applications: Measure differences in migration rates between treated and untreated cells.

Wound-healing assays based on (A) scratching, (B) stamping, (C) solid barrier, (D) liquid barrier, (E) droplet chemical assay, (F) microfluidic chemical assay, (G) electrical assay, and (H) laser ablation.

Transwell cell migration and invasion assay (Boyden chamber assay)

Transwell migration assays have been widely used for studying the motility of different types of cells including metastatic cancer cells. By using this method, we measure the capacity of cell motility and invasiveness toward a chemo-attractant gradient.

Suitable Applications: Measure effect of chemoattractant on migration rates

Other migration assays available

• Cell exclusion zone assay
• Fence assay (ring assay)
• Microcarrier bead assay
• Spheroid migration
• Capillary chamber migration assays (microfluidic chamber assays).

Other migration assays

Study Examples

Figure1: Results for wound-healing assays

Figure 2: Transwell migration and invasion assays of T24 and 5637 bladder urothelial cancer cells.

Quotations and ordering

Our customer service representatives are available 24hr a day!

References

1. Nina, K.; et al. In vitro cell migration and invasion assays. Mutation Research. 2013, 752: 10-24.
2. Calvin, R. Justus.; et al. In vitro cell migration and invasion assays. J Vis Exp. 2014, 88: e51046.

Explore Other Options

• Cell Viability Assays
• Cell Proliferation Assay Services
• Cell Apoptosis Assays
• Cell Angiogenesis Assays
• Cellular Phosphorylation Assays

For research use only. Not for any other purpose.