- You are here: Home
- Disease Models
- Urology Disease Models
- Renal Ischemia-Reperfusion Injury (RIRI) Model
Service
-
Cell Services
- Cell Line Authentication
- Cell Surface Marker Validation Service
-
Cell Line Testing and Assays
- Toxicology Assay
- Drug-Resistant Cell Models
- Cell Viability Assays
- Cell Proliferation Assays
- Cell Migration Assays
- Soft Agar Colony Formation Assay Service
- SRB Assay
- Cell Apoptosis Assays
- Cell Cycle Assays
- Cell Angiogenesis Assays
- DNA/RNA Extraction
- Custom Cell & Tissue Lysate Service
- Cellular Phosphorylation Assays
- Stability Testing
- Sterility Testing
- Endotoxin Detection and Removal
- Phagocytosis Assays
- Cell-Based Screening and Profiling Services
- 3D-Based Services
- Custom Cell Services
- Cell-based LNP Evaluation
-
Stem Cell Research
- iPSC Generation
- iPSC Characterization
-
iPSC Differentiation
- Neural Stem Cells Differentiation Service from iPSC
- Astrocyte Differentiation Service from iPSC
- Retinal Pigment Epithelium (RPE) Differentiation Service from iPSC
- Cardiomyocyte Differentiation Service from iPSC
- T Cell, NK Cell Differentiation Service from iPSC
- Hepatocyte Differentiation Service from iPSC
- Beta Cell Differentiation Service from iPSC
- Brain Organoid Differentiation Service from iPSC
- Cardiac Organoid Differentiation Service from iPSC
- Kidney Organoid Differentiation Service from iPSC
- GABAnergic Neuron Differentiation Service from iPSC
- Undifferentiated iPSC Detection
- iPSC Gene Editing
- iPSC Expanding Service
- MSC Services
- Stem Cell Assay Development and Screening
- Cell Immortalization
-
ISH/FISH Services
- In Situ Hybridization (ISH) & RNAscope Service
- Fluorescent In Situ Hybridization
- FISH Probe Design, Synthesis and Testing Service
-
FISH Applications
- Multicolor FISH (M-FISH) Analysis
- Chromosome Analysis of ES and iPS Cells
- RNA FISH in Plant Service
- Mouse Model and PDX Analysis (FISH)
- Cell Transplantation Analysis (FISH)
- In Situ Detection of CAR-T Cells & Oncolytic Viruses
- CAR-T/CAR-NK Target Assessment Service (ISH)
- ImmunoFISH Analysis (FISH+IHC)
- Splice Variant Analysis (FISH)
- Telomere Length Analysis (Q-FISH)
- Telomere Length Analysis (qPCR assay)
- FISH Analysis of Microorganisms
- Neoplasms FISH Analysis
- CARD-FISH for Environmental Microorganisms (FISH)
- FISH Quality Control Services
- QuantiGene Plex Assay
- Circulating Tumor Cell (CTC) FISH
- mtRNA Analysis (FISH)
- In Situ Detection of Chemokines/Cytokines
- In Situ Detection of Virus
- Transgene Mapping (FISH)
- Transgene Mapping (Locus Amplification & Sequencing)
- Stable Cell Line Genetic Stability Testing
- Genetic Stability Testing (Locus Amplification & Sequencing + ddPCR)
- Clonality Analysis Service (FISH)
- Karyotyping (G-banded) Service
- Animal Chromosome Analysis (G-banded) Service
- AAV Biodistribution Analysis (RNA ISH)
- Molecular Karyotyping (aCGH)
- Droplet Digital PCR (ddPCR) Service
- Digital ISH Image Quantification and Statistical Analysis
- SCE (Sister Chromatid Exchange) Analysis
- Biosample Services
- Histology Services
- Exosome Research Services
- In Vitro DMPK Services
-
In Vivo DMPK Services
- Pharmacokinetic and Toxicokinetic
- PK/PD Biomarker Analysis
- Bioavailability and Bioequivalence
- Bioanalytical Package
- Metabolite Profiling and Identification
- In Vivo Toxicity Study
- Mass Balance, Excretion and Expired Air Collection
- Administration Routes and Biofluid Sampling
- Quantitative Tissue Distribution
- Target Tissue Exposure
- In Vivo Blood-Brain-Barrier Assay
- Drug Toxicity Services
Renal Ischemia-Reperfusion Injury (RIRI) Model
Creative Bioarray boasts extensive expertise in conducting preclinical studies on acute kidney injury (AKI) through the use of rodent models. Our team has meticulously crafted a refined model for renal ischemia-reperfusion injury (RIRI), which stands out as a pivotal tool for the meticulous screening, rigorous testing, and comprehensive evaluation of your prospective drug candidates.
The RIRI model simulates the pathological sequence of ischemia/reperfusion injury (IRI), which initially involves a restriction in blood supply to the kidney, subsequently followed by the restoration of blood flow and re-oxygenation. This process, when occurring in the kidney, can lead to AKI, a clinical syndrome characterized by rapid kidney dysfunction and associated with high mortality rates. The cardinal factors that contribute to kidney damage during RIRI include oxidative stress, excessive accumulation of calcium in the cytosol, mitochondrial uncoupling, liberation of iron ions, and the activation of an inflammatory immune response. The RIRI model serves as a valuable tool for studying the mechanisms underlying AKI and evaluating potential therapeutic strategies for its prevention and treatment.
Fig. 1 Mechanisms of renal ischemia-reperfusion injury. (Krzywonos-Zawadzka et al. 2019)
Our Renal Ischemia-Reperfusion Injury Model
- Available Animal
- Rat
- Mouse
- Modeling Method
Animals are fasted for 8-12 hours before surgery. Following anesthesia, a midline incision is made starting from the xiphoid process. The abdominal cavity is then accessed, and the kidney is mobilized and released from its attachments. The renal artery is prepared for complete occlusion using surgical bulldog clips for 45-60 minutes to implement the ischemia-reperfusion protocol.
Fig. 2 Schematic diagram of the method for establishing the RIRI model
- Endpoints
- Serum analysis: BUN, creatinine, etc.
- Histology analysis
- Cytokine analysis
- qPCR or Western blot
- Other customized endpoints
Example Data
Fig. 3 Pioglitazone treatment improves renal IRI. (A) Serum creatinine levels in different groups at 24 h after renal IRI. (B) Serum urea nitrogen levels in different groups at 24 h after renal IRI. (Zou et al. 2021)
Quotation and Ordering
Creative Bioarray provides urological study services, focusing on conducting preclinical efficacy, proof-of-concept, and mechanism of action studies in different rodent models. Our scientists have the necessary skills and experience to evaluate the efficacy of your preclinical drug candidates. If you are interested in our services, please feel free to contact us at any time or submit an inquiry to us directly.
References
- Zou, G., et al. Pioglitazone ameliorates renal ischemia-reperfusion injury via inhibition of NF-κB activation and inflammation in rats. Frontiers in Physiology, 2021, 12: 707344.
- Krzywonos-Zawadzka, A., et al. Pharmacological protection of kidney grafts from cold perfusion-induced injury. BioMed Research International, 2019, 2019.
For research use only. Not for any other purpose.