In vitro Neurodermatology Cell Model

Skin and the central and/or peripheral nervous system share a common source (the ectoderm). Skin is the first interface between the human body and outside environment. Any variations in this environment like sun exposure, temperature change or any applications of substances on the skin can be detected by sensory neurons, and then an antidromic action potential is emitted which elicits neurotransmitters to be released, and then allows for an immediate response of the skin cells.

Creative Bioarray provides In vitro Neurodermatology cell model generated by co-culturing of sensory neurons and skin cells, which can better simulate in vivo human skin and provide innovative solutions to the dermo-pharmaceutical and cosmetic industries.

Creative Bioarray co-culture cell system

• Primary cultures of sensory neurons
• Co- culture of sensory neurons and keratinocytes
• Nerve-muscle co-culture
• Dorsal Root Ganglion explant with and without target
• The co-culture of keratinocytes and melanocytes

Specific Makers and measure endpoints

• Sensory neuron endings analysis and free nerve endings quantification
• Specific receptors (TRPV1, TrkA …)
• Model of nerve-muscle co-culture: spontaneous contractions of muscle fibers and analysis of myorelaxant effect
• Calcium mobilisation by sensory neurons
• Neurotransmitters released by the sensory neurons (CGRP, Substance P, …)
• Cytokines released by keratinocytes (IL-6, IL-8, IL- 1β)
• Neurotransmitters released by the sensory neurons (conditioned medium)
• Marked melanin produced by melanocytes
• Neurotransmitters released by the sensory neurons (CGRP , Substance P , …)
• Cytokine released by keratinocytes (IGF-1 , VEGF, bFGF … )
• Meurotransmitters released by the sensory neurons (CGRP, substance P)
• Cytokines released by keratinocytes (IL-6 , IL-8 , IL- 1β )
• Number of sensory neuron endings

Fig.1 Co-culture models containing dermal fibroblasts and keratinocytes were cultured for 13 days. (a–b) Neurite outgrowth induced by healthy keratinocytes. Neurites were stained for polyclonal anti-protein gene product 9.5 (PGP9.5) (red) and calcitonin gene-related peptide (CGRP) (green). Nuclei were detected with (DAPI) (blue). (c) A representative co-culture showing the proximity between DRG neurons and keratinocytes, immunostained with antibodies; anti-β-tubulin III for DRG (red), anti-MK14 for keratinocytes (green) and DAPI for nuclei (blue).  (d) Cells also expressed peripherin and Brn3a, markers of sensory neurons.

Applications

• Skin aging
• Inflammatory skin disorders
• Soothing
• Skin pigmentation
• Photo-aging
• Warming sensation
• Microcirculation
• Hair growth

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For research use only. Not for any other purpose.