Partial Sciatic Nerve Ligation (PSL/PSNL) Model
Creative Bioarray, a leading Contract Research Organization (CRO) specializing in pharmacological efficacy assessments, offers a robust and reliable partial sciatic nerve ligation (PSL/PSNL) model to our esteemed clients. This model is meticulously designed to facilitate the screening and evaluation of analgesic drugs, providing a comprehensive platform for testing the efficacy of potential pain-relieving compounds. Our commitment to excellence in preclinical research ensures that our clients have access to the most advanced models and methodologies, enabling them to make informed decisions in their drug development processes.
The management of chronic pain continues to be a significant challenge, with existing treatments often accompanied by undesirable side effects such as tolerance and addiction. Chronic neuropathic pain arises from damage or disease within the somatosensory system. In the quest for therapies with fewer adverse effects, animal models of pain are indispensable in preclinical research. The PSL/PSNL model is a method that generates chronic neuropathic pain in animals, manifesting as mechanical and thermal hypersensitivity, persistent pain, and alterations in limb temperature. This model is particularly well-suited for the preclinical study of neuropathic pain due to its ability to replicate many of the symptoms experienced by humans with this condition. Additionally, the surgical technique is relatively quick and simple to execute, making it a valuable tool in the investigation of neuropathic pain.
Our Partial Sciatic Nerve Ligation (PSL/PSNL) Model
- Available Animal
Rat
- Modeling Method
After anesthetization, an incision will be made in the skin and the muscle will be blunt dissected to expose the sciatic nerve. In the model group, the nerve is carefully freed and one-third to one-half of the nerve is tightly ligated with silk suture. In the sham group, the sciatic nerve is left intact and the muscle is sutured, and the skin closed with staples.
- Endpoints
- Behavioral tests: Von Frey test, Hot plate test, etc.
- Histology analysis
- Body weight
- Other customized endpoints
Example Data
Fig. 1 Effect of Azadirachta indica on PSNL-induced alterations in (A) Mechanical allodynia in von frey hair test, (B) Mechanical hyperalgesia in paw pressure test, (C) Thermal hyperalgesia in plantar test, (D) Thermal hyperalgesia in Hargreaves test, (E) fall off time in rotarod test and (F) Motor nerve conduction velocity. (Kandhare et al. 2017)
In addition, we also provide other neuropathic pain models that maybe you are interested in:
- Diabetes-Induced Neuropathic Pain Model
- Chemotherapy-Induced Neuropathic Pain Model
- Chronic Constriction Injury (CCI) Model
- Spared Nerve Injury (SNI) Model
- Spinal Nerve Ligation (SNL) Model
Quotation and Ordering
Creative Bioarray has a wealth of experience in conducting comprehensive efficacy studies for the meticulous screening and detailed profiling of drug candidates, employing a range of sophisticated preclinical pharmacological models specifically designed to simulate and study neuropathic pain. If you are interested in our services, please do not hesitate to contact us at any time or submit an inquiry to us directly.
References
- Kandhare, A.D., et al. Neuroprotective effect of Azadirachta indica standardized extract in partial sciatic nerve injury in rats: Evidence from anti-inflammatory, antioxidant and anti-apoptotic studies. EXCLI journal, 2017, 16: 546.
- Korah, H.E., et al. Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies. J Vis Exp. 2022;(188):10.3791/64555.
