Allergic Rhinitis (AR) Model

Boasting a wealth of expertise honed through years of rigorous research, Creative Bioarray proudly introduces our specialized rodent disease model tailored for allergic rhinitis (AR), alongside a comprehensive array of complementary services. Our dedicated team of scientists strives to enable groundbreaking discoveries and drive advancements in drug development through our offerings.

AR is caused by reactions to inhaled allergens mediated by immunoglobulin E (IgE). It is a common chronic condition worldwide and often occurs alongside asthma and conjunctivitis. AR is a global health problem that causes significant burden and disability. During the sensitization phase, allergens are taken up by dendritic cells in the nasal mucosa. This process leads to the production of allergen-specific immunoglobulin E (IgE) by plasma cells. The IgE binds to mast cells, basophils, memory allergen-specific type 2 T helper cells (TH2 cells), and IgE+ B cells. In individuals sensitized to the allergen, subsequent exposure triggers the activation of mast cells and basophils in the nasal mucosa. This activation results in the release of allergic mediators, causing acute symptoms of allergic rhinitis. Memory allergen-specific TH2 cells also produce cytokines, which lead to an inflammatory response characterized by eosinophil recruitment within a few hours. This inflammatory response causes more symptoms and changes in the functional aspects of the nasal mucosa, resembling chronic rhinitis.

Fig. 1 Pathophysiology of allergic rhinitis. (Bousquet et al. 2020)

Our Allergic Rhinitis Model

• Available Animal

Balb/c mouse

• Modeling Method

Mice are sensitized to ovalbumin (OVA) on days 0, 7, and 14 by an intraperitoneal (i.p.) injection. One week after the last injection, on day 21, the mice are challenged with OVA in the bilateral nasal cavities.

Fig. 2 Schematic diagram of OVA-induced allergic rhinitis model

• Endpoints

• Nasal symptom score
• Cytokine analysis: IL-4, IL-5, IL-13 etc.
• Histology analysis: H&E staining, IHC
• OVA-specific IgE analysis
• qPCR or Western blot
• Other customized endpoints

Example Data

Fig. 3 Effect of wogonin on OVA-specific IgE levels in serum of the allergic rhinitis (AR) mouse model. (Kim et al. 2018)

Fig. 4 Effects of wogonin on the filtration of eosinophils (arrows) into nasal mucosa of mice with allergic rhinitis (AR). (Kim et al. 2018)

Quotation and Ordering

Creative Bioarray stands as a visionary research institute and premier service provider in the realm of preclinical research. We are devoted to crafting top-tier disease models that propel preclinical research initiatives forward. Our mission is to expedite your drug development journey. If you are interested in our services, please feel free to contact us at any time or submit an inquiry to us directly.

References

1. Kim, K.A., et al. Anti-inflammatory effect of wogonin on allergic responses in ovalbumin-induced allergic rhinitis in the mouse. Allergy & Rhinology, 2018, 9: 2152656718764145.
2. Bousquet, J., et al. Allergic rhinitis. Nature Reviews Disease Primers, 2020, 6(1): 95.

For research use only. Not for any other purpose.