Blood Plasma Partitioning Assay

The traditional pharmacokinetic parameters are mainly obtained by measuring plasma samples. However, some drugs bind strongly to red blood cells (RBCs), while others bind strongly to plasma proteins. This significantly affects their distribution between blood and plasma, leading to erroneous calculations of pharmacokinetic parameters. Therefore, for compounds with a high blood-to-plasma ratio, studying the concentration in whole blood can yield more accurate pharmacokinetic parameters.

Creative Bioarray employs fresh blood samples from humans and various laboratory animals to evaluate a compound's whole blood stability, erythrocyte distribution, and plasma ratio across different species. We use advanced chromatographic separation technologies, mass spectrometry, and high-sensitivity immunoassay methods to ensure data accuracy and reliability.

Brief Protocol

Prepare fresh whole blood with EDTA-K₂ of desired species such as human, rat, mouse, dog, and monkey.
Incubate the compound with the whole blood at physiological temperature (37°C) for 60 minutes.
Separate plasma and RBC fractions by centrifugation.
Use LC-MS/MS to determine compound concentrations in plasma and RBCs.
Calculate the whole blood-plasma distribution ratio.
Analyze the data to understand the compound's distribution behavior.

Sample Requirements

Molecular formula, molecular weight, solubility information, and storage condition of the compound.
At least 50 μL of 10 mM in DMSO or 5 mg of the solid compound.

Our Blood Plasma Partitioning Assay Can Help You

Understand drug distribution: Gain insights into the drug's distribution within the body, determining concentrations within different tissues and organs to optimize drug design and efficacy.
Evaluate pharmacokinetic properties: Assist in assessing absorption, distribution, metabolism, and excretion processes within the body.
Predict drug interactions: Evaluate the interaction potential of drugs under multi-drug treatment conditions.
Forecast blood toxicity: Predict potential blood system toxicity of drugs, providing vital reference for drug safety assessment.
Guide drug design and optimization: Improve drug efficacy and reduce potential side effects through informed drug design optimization.
Guide clinical medication: Assist doctors in devising rational medication plans based on the specific conditions of patients to avoid side effects due to overdosage or underdosage.

Features of Our Blood Plasma Partitioning Assay Service

High Precision: Utilize advanced detection technologies and equipment to ensure accurate and reliable data.
Rapid Delivery: Employ automated processing systems for efficient handling of large sample volumes, shortening detection cycle times.
Customized Services: Provide custom detection solutions and service processes tailored to client needs.

FAQ

1. Can the analysis results reflect the actual in vivo situation?

Yes, under specific conditions, they can reflect drug distribution in the body, but practical application should combine with in vivo experimental data.

2. How does the plasma distribution ratio affect drug design?

The ratio can help understand in vivo distribution, optimizing drug dosage and administration frequency.

3. How is result accuracy ensured?

We use standardized operational processes and rigorous quality control measures to ensure high-standard results for every sample batch.

4. What types of drugs can be measured?

Any drug with suitable analytical/quantification methods can be measured; please consult our technical support team for specific requirements.

5. What results are provided?

We offer detailed data interpretation and test reports. For any additional needs, please consult our technical team.

Quotation and Ordering

If you have any special needs or questions regarding our services, please feel free to contact us. We look forward to cooperating with you in the future.

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For research use only. Not for any other purpose.