Carrageenan-Induced Paw Edema Model

Creative Bioarray provides comprehensive in vivo pharmacodynamics studies utilizing a well-established animal model known as the carrageenan-induced paw edema model. This model is particularly effective for swiftly evaluating the anti-inflammatory properties of your drug candidates. By employing this established methodology, researchers can efficiently evaluate the efficacy of potential drug candidates in reducing inflammation, thereby accelerating the drug development process and ensuring the rapid progression of drug candidates to further stages of clinical evaluation.

Inflammation serves as the body’s initial protective response against injuries caused by various factors, both internal and external. These factors may encompass physical damage, chemical substances, microbial attacks, and even cancer. To evaluate the anti-inflammatory properties of drugs in acute inflammation, researchers frequently employ a reliable method of inducing paw edema. This is achieved through the intraplantar injection of carrageenan, a polysaccharide derived from seaweed. This method promptly triggers swelling, pain sensitivity, and redness in the paw, which are induced by proinflammatory agents like histamine, bradykinin, tachykinin, reactive oxygen species, and reactive nitrogen species. In this specific model, inflammation reaches its peak within a 4-hour timeframe.

Our Carrageenan-Induced Paw Edema Model

• Available Animal

Rat

• Modeling Method

The right hind paw receives intraplantar injection of carrageenan to induce paw edema model.

Fig. 1 Schematic diagram of the modeling method of carrageenan-induced paw edema model

• Endpoints

• Image of paw
• Paw volume
• Inflammatory marker analysis
• Histology analysis
• qPCR or Western blot
• Other customized endpoints

Example Data

Fig. 2 Effects of Carr, Ind, and CAPE on paw volume of rats. A: Control, B: Carr + Ind, C: Carr, D: Carr + CAPE 10, E: Carr + CAPE 30. (Semis et al. 2021)

Quotation and Ordering

With our advanced preclinical technology platforms and skilled teams, Creative Bioarray offers a comprehensive range of services for in vivo efficacy studies. We guarantee exceptional quality results and efficient turnaround time. If you are interested in our services, please do not hesitate to contact us at any time or submit an inquiry to us directly.

Reference

1. Semis, H.S., et al. Investigation of the anti-inflammatory effects of caffeic acid phenethyl ester in a model of λ-Carrageenan–induced paw edema in rats. Human & experimental toxicology, 2021, 40(12_suppl): S721-S738.

For research use only. Not for any other purpose.