32D

Cat.No.: CSC-C1101

Species: Mouse

Source: bone marrow

Morphology: mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent

Culture Properties: suspension

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Cat.No.

CSC-C1101

Description

Established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus; cells are constitutively growth factor-dependent

Species

Mouse

Source

bone marrow

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS+10U/ml rmIL-3

Culture Properties

suspension

Morphology

mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent

Karyotype

Murine hypodiploid karyotype - 35(34-35)<2n> - carries 5 centric fusion markers

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD11b +, F4/80 (+)
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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