3T3-L1 Preadipocytes

Description: Preadipocytes are isolated from omental, mesenteric, or perirenal adipose tissue and are tested for their ability to differentiate in culture to mature adipocytes. Preadipocytes are available cryopreserved or plated and mature adipocytes are available in a variety of plated formats.
The greater omentum is the largest peritoneal fold within the abdomen and has an immunologic function. The omentum aids in isolating peritoneal infection and absorbing contaminants through the mesothelial stomata. Adipokines and fatty acids from this depot have direct access to the liver through the portal circulation which may lead to hepatic dysfunction.

Description: Preadipocytes are isolated from omental, mesenteric, or perirenal adipose tissue and are tested for their ability to differentiate in culture to mature adipocytes. Preadipocytes are available cryopreserved or plated and mature adipocytes are available in a variety of plated formats.
Perirenal adipose resides near the kidneys outside the peritoneal cavity. Fat from this depot has not been correlated with increased risk for metabolic disease.

Description: Visceral preadipocytes can be cultured as growing precursor cells or differentiated into adipocytes using medium supplemented with adipogenic and lipogenic hormones. This instruction manual describes procedures required to induce human preadipocytes to differentiate into mature adipocytes as well as culturing methods for human preadipocytes and adipocytes

Description: Adipocytes play an important role in energy storage and metabolism. Adipocyte differentiation is a developmental process that is critical for metabolic homeostasis and nutrient signaling. It is controlled by complex actions involving gene expression and signal transduction. Preadipocytes are present throughout adult life in adipose tissues and can proliferate and differentiate into mature adipocytes according to the energy balance. The proliferation and differentiation of these preadipocytes contribute to increases in adipose tissue mass. In vitro study indicates that different tissue-derived preadipocytes exhibit differently in lipid accumulation, adipogenic transcription factor expression, and TNF?-induced apoptosis. It has also been demonstrated that there is a close relationship between adipocyte differentiation and many physiological and pathological processes including fat metabolism, energy balance, obesity, diabetes, hyperlipidemia and breast cancer. HPA-s from Bioarray Research Laboratories are isolated from human subcutaneous fat tissue. HPA-s are cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HPA-s are characterized by immunofluorescent method with antibodies to CD44, CD90 and lipid staining after differentiation. HPA-s are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPA-s are guaranteed to further culture at the conditions provided by Bioarray Research Laboratories.

Description: Microvascular endothelial cells line blood vessels and contribute to many biological processes such as angiogenesis, coagulation, trafficking of lymphocytes, and the inflammatory response. Microvascular endothelial cells are diverse and have specific cellular characteristics and functions depending on the organ/tissue in which they are found. Adipose tissue is unique because it has the capacity to continually grow throughout adult life. It thus has a high occurrence of angiogenesis in order to provide the extensive vascularization require for adipose tissue. Studies have shown that angiogenesis precedes adipogenesis, implying that microvascular endothelial cells influence the proliferation of preadipocytes. Microvascular endothelial cell growth is at the same time stimulated by adipocyte secreted VEGG, suggesting a complex paracrine relationships between microvascular endothelial cells and preadipocytes during tissue development.HAMEC from Bioarray Research Laboratories are isolated from human adipose tissue. HAMEC are cryopreserved after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HAMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HAMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAMEC are guaranteed to further expand for 15 population doublings at the conditions provided by Bioarray Research Laboratories.

Description: Preadipocytes, found in adipose tissues throughout adulthood, can proliferate and differentiate into mature adipocytes based on energy balance, which in turn increases adipose tissue mass. In vitro studies show that preadipocytes from different tissues vary in lipid accumulation, expression of adipogenic transcription factors, and susceptibility to TNF-induced apoptosis. Furthermore, adipocyte differentiation is closely linked to various physiological and pathological processes, including fat metabolism, energy balance, obesity, diabetes, hyperlipidemia, and breast cancer.
Human Preadipocytes - Visceral (HPAs-v) from Cretive Bioarray are isolated from human visceral fat tissue. HPA-v are cryopreserved at passage one and delivered frozen. Each vial contains at least 0.5 x 10^6 cells in 1 ml volume. HPAs-v are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAs-v are guaranteed for further culture under conditions specified by Creative Bioarray.