T2

Cat.No.: CSC-C6231X

Species: Human

Source: somatic cell hybrid

Morphology: round to polymorphic cells growing in suspension singly and in clumps

Culture Properties: suspension

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C6231X

Description

Established by PEG-mediated fusion of the B-lymphoblastoid cell line (B-LCL) LCL 721.174 with an 8-azaguanine and ouabain-resistant variant of the T-LCL CEM (CEMR.3); cells were described to synthesize HLA-A2 and -B5 RNA, but express only small amounts of A2 and no B5 antigen on the cell surface; subclone of the T1 cell line which has lost both CEMR-derived copies of chromosome 6; cell line also known as 174xCEM.T2

Species

Human

Source

somatic cell hybrid

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

round to polymorphic cells growing in suspension singly and in clumps

Karyotype

Human flat-moded hypertriploid karyotype with 5% polyploidy; 69-78<3n>XX, +2, +11, +13, +14, +16, +20, +20, +21, +mar, add(X)(q11), del(7)(q31.3), add(8)(p11), t(8;9)(p11;p24), del(9)(q21)x1-2, add(14)(q32)x1-2, add(16)(q11), add(18)(q23); derived from CC

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD2 -, CD3 -, CD4 +, CD5 -, CD6 -, CD7 +, CD8 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 -, CD138 -, TCRalpha/beta -, TCRgamma/delta -, HLA-DR -
Viruses: PCR: EBV +, HBV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How can we ensure the integrity of tumor cell lines during quality control processes?

Integrity can be ensured by regularly authenticating cell lines, testing for mycoplasma contamination, and monitoring genetic stability.

Ask a Question

Average Rating: 5.0 | 1 Scientist has reviewed this product

Highly viable

The cells were not only pure but also highly viable, ensuring accurate and reliable experimental results. The supplier provides detailed protocols and documentation, allowing for seamless integration into my research workflow. Would reorder this product.

12 May 2023

Ease of use
After sales services
Value for money

Write your own review

You May Also Need

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

CAL-39

Description: Established from the primary lesion resected from a 76-year-old woman with bulky, moderately differentiated squamous cell carcinoma of the vulva in 1984; cells were reported to express an elevated number of high affinity EGF receptors, to carry gene amplification at 11q13 and to be tumorigenic in nude mice

Cat#: CSC-C0316 INQUIRY

View Product

EGI-1

Description: Established from the solid tumor of a 52-year-old Caucasian man with advanced stage malignant bile duct carcinoma (patient had not received any chemotherapy prior to cell explantation) in 1984 and three consecutive passages in female nu/nu mice (NMRI); primary tumor histology: large cell adenocarcinoma of low differentiation, metastasizing (ascites in patient); human character of cell line confirmed by karyotype in passage #34 of in vitro culture (1986)

Cat#: CSC-C0433 INQUIRY

View Product

AC-1M81

Description: Subclone of ACH1P which was established by fusion of the JEG-3-derived HGPRT-defective mutant cell line AC1-1 with primary male trophoblast cells isolated from term human chorion laeve; described as possessing both, a distinct karyotype and a distinct expression pattern of tumor-related genes

Cat#: CSC-C0485 INQUIRY

View Product

AC-1M88

Description: One of five phenotypically and cytogenetically distinct subclones of a hybrid (ACH1P) formed by fusing an HGPRT-defective mutant (AC1-1) of JEG-3 human choriocarcinoma with primary human trophoblast (chorion laeve) cells

Cat#: CSC-C0486 INQUIRY

View Product

ACH1P

Description: Established by somatic hybridization of primary male trophoblast cells and the HGPRT-defective choriocarcinoma cell line AC1-1 which was originally derived from cell line JEG-3

Cat#: CSC-C0496 INQUIRY

View Product

NTERA-2

Description: NTERA-2 was cloned from cell line TERA-2 which was derived from a metastatic teratocarcinoma of a 22-year-old Caucasian male; cell line also known as NT-2

Cat#: CSC-C0535 INQUIRY

View Product

For research use only. Not for any other purpose.