EGI-1

Cat.No.: CSC-C0433

Species: Human

Source: bile duct carcinoma

Morphology: adherent polymorphic (epitheloid or dendritric) cells growing in monolayers with multilayer foci

Culture Properties: monolayer

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Cat.No.

CSC-C0433

Description

Established from the solid tumor of a 52-year-old Caucasian man with advanced stage malignant bile duct carcinoma (patient had not received any chemotherapy prior to cell explantation) in 1984 and three consecutive passages in female nu/nu mice (NMRI); primary tumor histology: large cell adenocarcinoma of low differentiation, metastasizing (ascites in patient); human character of cell line confirmed by karyotype in passage #34 of in vitro culture (1986)

Species

Human

Source

bile duct carcinoma

Recommended Medium

90% DMEM + 10% h.i. FBS + 1x MEM amino acids (both essential and non-essential) + 2 mM L-glutamine

Culture Properties

monolayer

Morphology

adherent polymorphic (epitheloid or dendritric) cells growing in monolayers with multilayer foci

Karyotype

Human hypertriploid with 8% polyploidy - 70(68-72)<3n>XYY, -8, -10, +11, -12, -15, +21, +5mar, del(4)(q22), i(5q), i(6p)x2, add(18)(q23)x2, del(21)(q22) - rearrangements of 18q and 21q implicated in gall bladder carcinoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vime

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the storage and stability requirements for EMEM?

EMEM should be stored in a cool, dry place and protected from light. Proper storage conditions help maintain the stability of the medium and its components.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Reliable applications

I've used this cell product for gene expression studies. The cells showed the expected gene signatures, allowing me to analyze molecular pathways accurately.

8 Oct 2023

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For research use only. Not for any other purpose.