NTERA-2

Cat.No.: CSC-C0535

Species: Human

Source: embryonal carcinoma (teratocarcinoma)

Morphology: adherent epitheloid cells carrying strong granula growing adherent in monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0535

Description

NTERA-2 was cloned from cell line TERA-2 which was derived from a metastatic teratocarcinoma of a 22-year-old Caucasian male; cell line also known as NT-2

Species

Human

Source

embryonal carcinoma (teratocarcinoma)

Recommended Medium

85% DMEM + 10% h.i.FBS + 5% horse serum.

Culture Properties

monolayer

Morphology

adherent epitheloid cells carrying strong granula growing adherent in monolayer

Karyotype

Human flat-moded hyperdiploid karyotype - 48-58<2n>XXXY, +1, +16, +20, +22, +3-4mar, add(X)(p11), add(X)(q2?5), der(X;1)(p10;q10), add(1)(p35-36), add(11)(p15), add(13)(q3?1) - sideline with i(3q)

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Viruses: PCR:

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Can EMEM be used for long-term cell culture maintenance?

Yes, EMEM can be used for long-term cell culture maintenance when supplemented with serum or other additives to support cell growth and viability over extended periods.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Easy to culture

The cells were easy to grow and expanded well, giving me enough to work with.

08 Nov 2023

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For research use only. Not for any other purpose.