AC-1M81

Cat.No.: CSC-C0485

Species: Human

Source: choriocarcinoma-trophoblast hybrid

Morphology: epithelial-like adherent cells growing in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0485

Description

Subclone of ACH1P which was established by fusion of the JEG-3-derived HGPRT-defective mutant cell line AC1-1 with primary male trophoblast cells isolated from term human chorion laeve; described as possessing both, a distinct karyotype and a distinct expression pattern of tumor-related genes

Species

Human

Source

choriocarcinoma-trophoblast hybrid

Recommended Medium

Ham's F-12 and 10% h.i. FBS.

Culture Properties

monolayer

Morphology

epithelial-like adherent cells growing in monolayers

Karyotype

Human flat-moded hypertetraploid karyotype with 4% polyploidy - 80-106<4n>XX, -Y, -3, -4, +7, +9, -13, -18, +22, +4-6mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?->4p1?::11q1?1->11qter), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32)x2, i(13q), add(15)

Quality Control

Mycoplasma: contamination was eliminated with sparfloxacin, then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the advantages of using EMEM in cell culture?

EMEM provides a stable and well-defined environment for cell growth, making it suitable for both research and industrial applications. It can support the growth of cells with minimal adaptation.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Exceptional performance

We have experienced unparalleled success in our cellular applications with the incorporation of these products, benefitting from their exceptional performance and compatibility.

09 Feb 2023

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For research use only. Not for any other purpose.