AC-1M88

Cat.No.: CSC-C0486

Species: Human

Source: choriocarcinoma-trophoblast hybrid

Morphology: epitheloid cells growing adherently in monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0486

Description

One of five phenotypically and cytogenetically distinct subclones of a hybrid (ACH1P) formed by fusing an HGPRT-defective mutant (AC1-1) of JEG-3 human choriocarcinoma with primary human trophoblast (chorion laeve) cells

Species

Human

Source

choriocarcinoma-trophoblast hybrid

Recommended Medium

Ham's F-12 and 10% h.i. FBS.

Culture Properties

monolayer

Morphology

epitheloid cells growing adherently in monolayers

Karyotype

Human flat-moded hypertetraploid karyotype with 11% polyploidy - 80-99<4n>XXX, -Y, -3, +7, +7, -8, +9, +9, +12, -13, +3-5mar, del(X)(p11p22), der(7)t(7;7)(p22;q11.22)add(7)(q32)x2, add(7)(q32), add(9)(p24), i(13q), add(19)(p13), der(19)t(11;19)(q11;q13) -

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin +
Viruses: PCR: EBV -, HBV -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How does EMEM compare to other cell culture media?

EMEM is similar to other basal media such as DMEM (Dulbecco's Modified Eagle's Medium) but may have slightly different nutrient compositions and applications.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Effective study

I purchased this cell product for immunological studies. The cells provided were highly responsive to immune stimuli, allowing me to effectively study the dynamics of the immune system.

09 Feb 2023

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For research use only. Not for any other purpose.