Human Adipose Microvascular Endothelial Cells (HAMEC)

Description: Preadipocytes are isolated from omental, mesenteric, or perirenal adipose tissue and are tested for their ability to differentiate in culture to mature adipocytes. Preadipocytes are available cryopreserved or plated and mature adipocytes are available in a variety of plated formats.
The greater omentum is the largest peritoneal fold within the abdomen and has an immunologic function. The omentum aids in isolating peritoneal infection and absorbing contaminants through the mesothelial stomata. Adipokines and fatty acids from this depot have direct access to the liver through the portal circulation which may lead to hepatic dysfunction.

Description: Preadipocytes are isolated from omental, mesenteric, or perirenal adipose tissue and are tested for their ability to differentiate in culture to mature adipocytes. Preadipocytes are available cryopreserved or plated and mature adipocytes are available in a variety of plated formats.
Perirenal adipose resides near the kidneys outside the peritoneal cavity. Fat from this depot has not been correlated with increased risk for metabolic disease.

Description: Visceral preadipocytes can be cultured as growing precursor cells or differentiated into adipocytes using medium supplemented with adipogenic and lipogenic hormones. This instruction manual describes procedures required to induce human preadipocytes to differentiate into mature adipocytes as well as culturing methods for human preadipocytes and adipocytes

Description: 3T3-L1 murine adipocytes have been fundamental in metabolic disease research for over 30 years. Originally derived from Swiss mouse embryo tissue by Dr. Howard Green of Harvard Medical School, the 3T3-L1 system has been pivotal in advancing the understanding of basic cellular mechanisms associated with diabetes, obesity and related disorders.
We now offer both Cryopreserved and Plated sub-confluent 3T3-L1 preadipocytes in 96 well format and media for proliferation, differentiation, maintenance of 3T3-L1 preadipocytes to adipocytes. We also offer media and reagent kits validated using 3T3-L1 and/or primary rodent preadipocytes and adipocytes. High quality control tested 3T3-L1 preadipocytes designed to work consistently using Bioarray's line of 3T3-L1 media.

Description: Adipocytes play an important role in energy storage and metabolism. Adipocyte differentiation is a developmental process that is critical for metabolic homeostasis and nutrient signaling. It is controlled by complex actions involving gene expression and signal transduction. Preadipocytes are present throughout adult life in adipose tissues and can proliferate and differentiate into mature adipocytes according to the energy balance. The proliferation and differentiation of these preadipocytes contribute to increases in adipose tissue mass. In vitro study indicates that different tissue-derived preadipocytes exhibit differently in lipid accumulation, adipogenic transcription factor expression, and TNF?-induced apoptosis. It has also been demonstrated that there is a close relationship between adipocyte differentiation and many physiological and pathological processes including fat metabolism, energy balance, obesity, diabetes, hyperlipidemia and breast cancer. HPA-s from Bioarray Research Laboratories are isolated from human subcutaneous fat tissue. HPA-s are cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HPA-s are characterized by immunofluorescent method with antibodies to CD44, CD90 and lipid staining after differentiation. HPA-s are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPA-s are guaranteed to further culture at the conditions provided by Bioarray Research Laboratories.

Description: Preadipocytes, found in adipose tissues throughout adulthood, can proliferate and differentiate into mature adipocytes based on energy balance, which in turn increases adipose tissue mass. In vitro studies show that preadipocytes from different tissues vary in lipid accumulation, expression of adipogenic transcription factors, and susceptibility to TNF-induced apoptosis. Furthermore, adipocyte differentiation is closely linked to various physiological and pathological processes, including fat metabolism, energy balance, obesity, diabetes, hyperlipidemia, and breast cancer.
Human Preadipocytes - Visceral (HPAs-v) from Cretive Bioarray are isolated from human visceral fat tissue. HPA-v are cryopreserved at passage one and delivered frozen. Each vial contains at least 0.5 x 10^6 cells in 1 ml volume. HPAs-v are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAs-v are guaranteed for further culture under conditions specified by Creative Bioarray.