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BEAS-2B
Cat.No.: CSC-C9087W
Species: Human
Source: Bronchus
Morphology: epithelial
Cell Type: Epithelial
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BEAS-2B cells are a widely utilized immortalized, non-tumorigenic human cell line, established in 1988 from a bronchial epithelial biopsy of a healthy male, lacking cancerous pathology. This cell line was immortalised by infection and cloning with the adenovirus 12-SV40 hybrid virus while maintaining the potential to differentiate into squamous tissue when exposed to serum. BEAS-2B cells also encode many lung epithelial cell markers, including cytokeratin, E-cadherin and alpha-smooth muscle actin (α-SMA). BEAS-2B cells in serum-free medium have the typical polygonal shape of respiratory epithelial cells, while those in serum-rich media become more fibroblastic at lower cell counts.
BEAS-2B cell line has been instrumental in numerous areas of research. In addition to being used as an in vitro model of lung epithelial cells to test chemical and pharmaceutical toxicology, it is also used for the analysis of lung cancer, asthma and chronic obstructive pulmonary disease (COPD). For viral infection, BEAS-2B cells are routinely employed to determine the infectivity and pathogenicity of respiratory viruses, including respiratory syncytial virus. Furthermore, as an immortalised cell line, BEAS-2B cells are very abundant and transfectible, making them ideal targets for genetic editing. Scientists use technologies like CRISPR/Cas9 gene knockout, knock-in or point mutations on BEAS-2B cells to examine the functioning of genes and the underlying pathologies. All in all, the cellular anatomy, location and function of BEAS-2B cells make them an essential tool for studies on lung tumours, viral infections, and drug metabolism.
Fig. 1. Light micrographic findings of attached Beas-2B cells (H&E staining) (Lee, Y. and Ryu, Y. J., 2023).
Cytotoxic and ROS-Inducing Effects of NH2-PS-MPs in BEAS-2B Cells
Microplastics are small, persistent particles that can enter the human body through inhalation and penetrate deep into the lungs. While a number of studies have shown their inhalation toxicity, the findings are still controversial.
Jeon's team tested the inhalation toxicity of three different-charged PS-MPs on human bronchial epithelial cells (BEAS-2B) and on animals. PS-MPs were evaluated for their cytotoxic properties by WST-1 and MTT, in which BEAS-2B cells were exposed to 25–400 g/ml PS-MPs, COOH-PS-MPs, and NH2-PS-MPs for 24 hours. Only NH2-PS-MPs showed dose-dependent cytotoxicity with lower cell viability, a result consistent between the assays, especially at 200 g/ml. In addition, reactive oxygen species (ROS) production was measured by oxidised DCFH-DA levels, with hydrogen peroxide as a positive control. These measurements demonstrated a dramatic rise in ROS from BEAS-2B cells exposed to NH2-PS-MPs over PS-MPs or COOH-PS-MPs, as well as an impressive surge after 24 hours under 100 and 200 g/ml NH2-PS-MPs. These findings revealed that only the positively charged PS-MPs (NH2-PS-MPs) were cytotoxic and also produced more ROS in BEAS-2B cells.
Fig. 1. Cytotoxicity of various PS-MPs were measured using (A) WST-1 assay and (B) MTT assay (Jeon, M. S., Kim, J. W., et al., 2023).
Fig. 2. Reactive oxygen species (ROS) generated by various PS-MPs were measured using DCF-DA assay (Jeon, M. S., Kim, J. W., et al., 2023)
Effects Of Cadmium on Apoptosis, ROS Levels, and Mitochondrial Membrane Potential in BEAS-2B
Cadmium, a harmful heavy metal pollutant from industrial activities, posing risks for diseases such as asthma, lung cancer, and emphysema. Cao's team examined cadmium's toxicity on human bronchial epithelial cells (BEAS-2B). Results show decreased cell viability, increased ROS, and enhanced apoptosis via mitochondria-mediated intrinsic apoptosis pathway and MAPK signaling activation, providing insights into cadmium-induced lung disease mechanisms.
Hoechst33258 is a fluorescent marker that detects cell death. When cadmium was added to BEAS-2B cells in the following amounts (20, 40 and 60 M), hoechst33258 fluoresced brighter, and the fluorescent spots were generally more widely distributed. The fluorescence intensity of cadmium-treated samples was 4.34, 5.21 and 5.92 times that of control sample respectively. The ROS measurement showed that the fluorescence intensity of the cadmium-treated mice was 2.78, 4.20, and 4.68 times higher than that of the control; intracellular ROS increased dose-dependently as cadmium levels increased. These mitochondrial membrane potential assay results showed that cadmium treatment fluorescence was 0.83, 0.37 and 0.34 times stronger than the control (dose-dependently decreasing fluorescence intensity following cadmium treatment).
Fig. 3. The effects of cadmium on cell apoptosis (A-B) ROS levels (C-D), and mitochondrial membrane potential (E-F) of BEAS-2B cells (Cao, X., Fu, M., et al., 2021).
BEAS-2B is an immortalized but non-tumorigenic epithelial cell line from human bronchial epithelium. BEAS-2B cell line has been widely used as an in vitro cell model in a large variety of studies associated with respiratory diseases including lung carcinogenesis.
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20 Feb 2023
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Preferred supplier
Creative Bioarray’s professionalism and product quality make it my preferred supplier.
20 Feb 2023
Ease of use
After sales services
Value for money
Preferred supplier
Creative Bioarray’s professionalism and product quality make it my preferred supplier.
20 Feb 2023
Ease of use
After sales services
Value for money
Preferred supplier
Creative Bioarray’s professionalism and product quality make it my preferred supplier.
20 Feb 2023
Ease of use
After sales services
Value for money
Preferred supplier
Creative Bioarray’s professionalism and product quality make it my preferred supplier.
20 Feb 2023
Ease of use
After sales services
Value for money
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Description: The most abundant cell type in the bronchus is fibroblasts. They resemble a mesenchymal stem cell phenotype and their principle function is the production of type III collagen, elastin, and proteoglycans of the extracellular matrix. Bronchial fibroblasts play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial for effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts can result in abnormal tissue function. For example, the excess fibroblast proliferation and collagen secretion that occurs from bronchial subepithelial fibrosis can result in airway obstruction and bronchial hyper-responsiveness.
HBF are isolated from human bronchus tissue. HBF are cryopreserved at secondary culture (P1) and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBF are characterized by immunofluorescent method with antibody to fibronectin. HBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBF are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Description: The epithelium serves as the interface between the body and the external environment, covering all external surfaces and internal lumina. Epithelial cells, depending on their tissue of origin, perform various functions such as absorption, secretion, protection, transcellular transport, and sensation.
Human Tracheal Epithelial Cells (HTECs) from Creative Bioarray are isolated from the surface epithelium of healthy human tracheas, cryopreserved at passage 1, and shipped frozen. Each vial contains a minimum of 0.5 x 10^6 cells. These cells are tested and confirmed negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. They are guaranteed to achieve at least 12 population doublings under the conditions specified by Creative Bioarray.
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Creative Bioarray complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established.
Description: Creative Bioarray's normal Human Bronchial/Tracheal Epithelial Cells, when grown in Creative Bioarray's LIBro Medium, provide an ideal serum-free culture model for the accurate studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing. Creative Bioarray's HBTEC are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HBTEC are quality tested in LIBro B/T Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media.
Cell Features:
HBTEC are cryopreserved as primary cells. Cells are isolated from human bronchi/trachea and expanded once in culture vessels before being harvested for cryopreservation.
HSAEC are cryopreserved after primary cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation.
Creative Bioarray's Human Airway Epithelial Cells are not exposed to retinoic acid during isolation or expansion.
Human Airway Epithelial Cells are extensively tested for quality and optimal performance.
Creative Bioarray guarantees performance and quality.
Description: Human Bronchial Epithelial Cells are isolated from normal human bronchial tissue.
Description: Human Primary Tracheal Fibroblasts are isolated from normal human tracheal tissue. Human Primary Tracheal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray' Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml.
