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BEAS-2B
Cat.No.: CSC-C9087W
Species: Human
Source: Bronchus
Morphology: epithelial
Cell Type: Epithelial
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BEAS-2B cells are a widely used immortalized but non-tumorigenic human cell line, established in 1988 from the bronchial epithelial biopsy of a normal male individual without cancer. This cell line was immortalized through infection and cloning with the adenovirus 12-SV40 hybrid virus, retaining the ability to undergo squamous differentiation in response to serum. BEAS-2B cells express various lung epithelial cell markers such as cytokeratin, E-cadherin, and α-smooth muscle actin (α-SMA). In serum-free medium, BEAS-2B cells exhibit the typical polygonal morphology of respiratory epithelial cells; however, in serum-containing medium, the cells may become more fibroblastic, especially at lower cell densities.
BEAS-2B cell line has been instrumental in numerous areas of research. It serves not only as an in vitro model for lung epithelial cells to evaluate the toxic effects of chemicals and drugs but also plays a significant role in the study of respiratory diseases, including lung cancer, asthma, and chronic obstructive pulmonary disease. In the context of viral infection mechanisms, BEAS-2B cells are extensively used to investigate the infection mechanisms and pathogenicity of respiratory viruses such as respiratory syncytial virus. Additionally, as an immortalized cell line, BEAS-2B cells possess high proliferative and transfection efficiencies, making them suitable for genetic editing. Researchers employ technologies such as CRISPR/Cas9 for gene knockout, gene knock-in, or point mutations in BEAS-2B cells to study gene functions and the mechanisms of disease. In summary, the unique cellular morphology, location, and function of BEAS-2B cells make them an essential tool for investigating topics such as respiratory system tumors, viral infections, and drug metabolism activation.
Fig. 1. Light micrographic findings of attached Beas-2B cells (H&E staining) (Lee, Y. and Ryu, Y. J., 2023).
Cytotoxic and ROS-Inducing Effects of NH2-PS-MPs in BEAS-2B Cells
Microplastics are small, persistent particles that can enter the human body through inhalation and penetrate deep into the lungs. Although several studies have reported their inhalation toxicity, the findings remain controversial.
Jeon's team investigated the inhalation toxicities of three differently charged polystyrene microplastics (PS-MPs) using human bronchial epithelial cells (BEAS-2B) and animal models. The cytotoxic effects of PS-MPs were assessed using WST-1 and MTT assays, where BEAS-2B cells were treated with 25–400 μg/ml concentrations of PS-MPs, COOH-PS-MPs, and NH2-PS-MPs for 24 hours. Only NH2-PS-MPs exhibited significant dose-dependent cytotoxicity, as evidenced by reduced cell viability, which was consistent across both assays, particularly at 200 μg/ml. Additionally, reactive oxygen species (ROS) production was evaluated by measuring oxidized DCFH-DA levels, with hydrogen peroxide as the positive control. Results showed a significantly higher increase in ROS in BEAS-2B cells treated with NH2-PS-MPs compared to PS-MPs or COOH-PS-MPs, with notable elevations observed after 24-hour exposure to 100 and 200 μg/ml concentrations of NH2-PS-MPs (p < .05). The above results indicated that only the positively charged PS-MPs (NH2-PS-MPs) showed cytotoxicity and increased ROS generation in BEAS-2B cells.
Fig. 1. Cytotoxicity of various PS-MPs were measured using (A) WST-1 assay and (B) MTT assay (Jeon, M. S., Kim, J. W., et al., 2023).
Fig. 2. Reactive oxygen species (ROS) generated by various PS-MPs were measured using DCF-DA assay (Jeon, M. S., Kim, J. W., et al., 2023)
Effects Of Cadmium on Apoptosis, ROS Levels, and Mitochondrial Membrane Potential in BEAS-2B
Cadmium, a harmful heavy metal pollutant from industrial activities, posing risks for diseases such as asthma, lung cancer, and emphysema. Cao's team examined cadmium's toxicity on human bronchial epithelial cells (BEAS-2B). Results show decreased cell viability, increased ROS, and enhanced apoptosis via mitochondria-mediated intrinsic apoptosis pathway and MAPK signaling activation, providing insights into cadmium-induced lung disease mechanisms.
Hoechst33258 is a fluorescent dye used for detecting cell apoptosis. After treating BEAS-2B cells with different concentrations of cadmium (20, 40, and 60 μM), the fluorescence intensity of hoechst33258 increased, and the fluorescent spots tended to be uniformly distributed. The fluorescence intensity in the cadmium-treated groups was 4.34, 5.21, and 5.92 times higher than the control group, respectively. ROS detection results indicated that the fluorescence intensity in the cadmium-treated groups was 2.78, 4.20, and 4.68 times higher than the control, showing a dose-dependent increase in intracellular ROS levels with rising cadmium concentrations. Mitochondrial membrane potential assay results showed that the fluorescence intensity in cadmium-treated groups was 0.83, 0.37, and 0.34 times that of the control, demonstrating a dose-dependent decrease in fluorescence intensity after cadmium treatment.
Fig. 3. The effects of cadmium on cell apoptosis (A-B) ROS levels (C-D), and mitochondrial membrane potential (E-F) of BEAS-2B cells (Cao, X., Fu, M., et al., 2021).
BEAS-2B is an immortalized but non-tumorigenic epithelial cell line from human bronchial epithelium. BEAS-2B cell line has been widely used as an in vitro cell model in a large variety of studies associated with respiratory diseases including lung carcinogenesis.
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Average Rating: 5.0 | 5 Scientist has reviewed this product
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Creative Bioarray’s professionalism and product quality make it my preferred supplier.
20 Feb 2023
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Preferred supplier
Creative Bioarray’s professionalism and product quality make it my preferred supplier.
20 Feb 2023
Ease of use
After sales services
Value for money
Preferred supplier
Creative Bioarray’s professionalism and product quality make it my preferred supplier.
20 Feb 2023
Ease of use
After sales services
Value for money
Preferred supplier
Creative Bioarray’s professionalism and product quality make it my preferred supplier.
20 Feb 2023
Ease of use
After sales services
Value for money
Preferred supplier
Creative Bioarray’s professionalism and product quality make it my preferred supplier.
20 Feb 2023
Ease of use
After sales services
Value for money
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Description: The most abundant cell type in the bronchus is fibroblasts. They resemble a mesenchymal stem cell phenotype and their principle function is the production of type III collagen, elastin, and proteoglycans of the extracellular matrix. Bronchial fibroblasts play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial for effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts can result in abnormal tissue function. For example, the excess fibroblast proliferation and collagen secretion that occurs from bronchial subepithelial fibrosis can result in airway obstruction and bronchial hyper-responsiveness.
HBF are isolated from human bronchus tissue. HBF are cryopreserved at secondary culture (P1) and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBF are characterized by immunofluorescent method with antibody to fibronectin. HBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBF are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Description: The epithelium serves as the interface between the body and the external environment, covering all external surfaces and internal lumina. Epithelial cells, depending on their tissue of origin, perform various functions such as absorption, secretion, protection, transcellular transport, and sensation.
Human Tracheal Epithelial Cells (HTECs) from Creative Bioarray are isolated from the surface epithelium of healthy human tracheas, cryopreserved at passage 1, and shipped frozen. Each vial contains a minimum of 0.5 x 10^6 cells. These cells are tested and confirmed negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. They are guaranteed to achieve at least 12 population doublings under the conditions specified by Creative Bioarray.
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Creative Bioarray complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established.
Description: Creative Bioarray's normal Human Bronchial/Tracheal Epithelial Cells, when grown in Creative Bioarray's LIBro Medium, provide an ideal serum-free culture model for the accurate studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing. Creative Bioarray's HBTEC are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HBTEC are quality tested in LIBro B/T Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media.
Cell Features:
HBTEC are cryopreserved as primary cells. Cells are isolated from human bronchi/trachea and expanded once in culture vessels before being harvested for cryopreservation.
HSAEC are cryopreserved after primary cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation.
Creative Bioarray's Human Airway Epithelial Cells are not exposed to retinoic acid during isolation or expansion.
Human Airway Epithelial Cells are extensively tested for quality and optimal performance.
Creative Bioarray guarantees performance and quality.
Description: Human Bronchial Epithelial Cells are isolated from normal human bronchial tissue.
Description: Human Primary Tracheal Fibroblasts are isolated from normal human tracheal tissue. Human Primary Tracheal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml.
