HEP-3B

Cat.No.: CSC-C0245

Species: Homo sapiens, human

Source: Liver

Morphology: epithelial-like cells growing as monolayers

Culture Properties: Monolayer

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Cat.No.

CSC-C0245

Description

Established from the tumor tissue of an 8-year-old black boy in 1976.The cells contain a 2.3 kb integrated hepatitis B virus genome fragment. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. Cells were described to produce a variety of proteins, e.g. alpha-fetoprotein, albumin, transferrin, alpha2-macroglobulin, alpha1-antitrypsin, haptoglobin and others.

Species

Homo sapiens, human

Source

Liver

Recommended Medium

EMEM + 10% h.i. FBS

Culture Properties

Monolayer

Morphology

epithelial-like cells growing as monolayers

STR DNA Profile

Amelogenin: X
CSF1PO: 8
D13S317: 12,14
D16S539: 10
D5S818: 13
D7S820: 8,10
THO1: 6,7
TPOX: 9
vWA: 17"

Karyotype

Human hypotriploid stemline with near tetraploid side line (6%) - 63 (60-64)<3n>XX, -Y, -1, -1, -2, -3, -4, -8, -9, -13, +der(11)t(1;11)(q11;q14)x2, der(?15)t(1;?15)(q11;q24), i(7p)

Applications

The HEP-3B cell line can be used as a suitable transfection host

Quality Control

Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 (+), cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, e

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x106 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What should I pay attention to when adding PBS in the cell culture process?

Pay attention to the wall to add to the edge of the culture dish, do not add PBS directly to the cells, it is easy to blow up all the cells.

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