CA-46

Cat.No.: CSC-C0234

Species: Homo sapiens (human)

Morphology: single cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0234

Description

Established from the ascites fluid of a patient with American-type Burkitt lymphoma; cells were described to be EBNA-negative, to carry the t(8;14) and to express BCL2 and MYC mRNA

Species

Homo sapiens (human)

Tissue of Origin

Lymphoblast

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single cells in suspension

Karyotype

Human near diploid karyotype with about 3% triploidy - 46(45-48)<2n>X/XY, dup(1)(q21q32), dup(7)(q12q22), t(8;14)(q24;q32) - additional rearrangements were present in a sideline, viz. t(6;13)(p21;q32) and del(11)(p11) - a supernumerary dmin was present in

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -
Viruses: ELISA: revers

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 9 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

How does pH affect cell culture?

Changes in pH in the cell culture environment can alter virtually every cellular process like metabolism, cell growth, and membrane potential. Extracellular pH is slightly alkaline and typically 7.3-7.4, while intracellular pH is slightly lower at 7.2.

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