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Human Bronchial Epithelial Cells
Cat.No.: CSC-C4872L
Species: Human
Source: Bronchus
Cell Type: Epithelial
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Never can cryopreserved cells be kept at -20 °C.
Human bronchial epithelial cells (HBECs) are part of the respiratory system, covering the surface of bronchi and comprising a key component of the airway epithelium, which is essential for the functioning of the respiratory system. HBECs have many different cell types, but are most commonly classified into three ultrastructural, functional and biochemical groups: basal, ciliated and secretory cells. Basal epithelial cells are cells found in the basal layer of the bronchial epithelium, typically cone-shaped and small in size. They are stem or primary cells of the epithelial cell layer, responsible for tissue repair and regeneration. Other bioactive molecules emitted by basal epithelial cells include neutral endopeptidase, 15-lipoxygenase derivatives and cytokines. Ciliated epithelial cells are abundant in the bronchial epithelium and columnar, with dense cilia lining the free surface. A cluster of mitochondria around the tip of the cell gives the power to drive the cilia directionally beat in order to flush mucus out of the airways. Secretory (goblet) cells are primarily responsible for releasing mucus and other bioactive materials that are necessary to keep airways moist, shielded and protected. Such cells also release bioactive molecules that have antimicrobial, anti-inflammatory or immunomodulatory effects. In addition, secretory cells have the capacity to self-renew and grow into ciliated epithelial cells. Because of their specialised shape and function, the cultivation of human bronchial epithelial cells provides an excellent vehicle for studying physiological mechanisms of cell proliferation, differentiation, mucus production and ciliary activity. They are therefore a wonderful in vitro model for studying the physiological and pathological functions of the respiratory system.
Fig. 1. Illustration of normal human bronchial epithelium (Bucchieri F., 2012).
Single-Channel Activity in Mitochondria of Human Bronchial Epithelial Cells
Mitochondrial potassium channels, including large-conductance Ca2+-regulated potassium channels (mitoBKca), play crucial roles in maintaining ion homeostasis, affecting mitochondrial respiration, and protecting cells from oxidative stress. Sek et al. demonstrated for the first time the functional properties of mitoBKca in the inner mitochondrial membrane of a human bronchial epithelial cell line. They identified two populations of mitoBKCa channels and the single-channel activity were characterized using the patch-clamp technique.
The first channel's opening probability (Po) increased from 18% at -60 mV to 95% at positive voltages. The second channel had a high Po at all potentials, rising from 72% at negative to 98% at + 60 mV, all in 100 µM Ca2+ (Fig. 1b). Control activity data fitted a Boltzmann function, yielding a V1/2 value of -38.4 mV (Fig. 2b). The control channel was recorded twice as often as the high-activity channel (Fig. 1c). Both were observed in the same mitochondrial isolations, showing no significant conductance differences: control channel at 286.2 ± 5.5 pS and high activity at 287.8 ± 2.8 pS, calculated from the current-voltage relationship (Fig. 2a). Both channels showed no current rectification. Closed and open dwell times were analyzed; high-activity channel closure times were about 1 ms (Fig. 2d), and in the control channel, it decreased from 15 ms at -60 mV to 2 ms at +60 mV (Fig. 2c). Both channel types showed increased open dwell times from -60 to +60 mV, from 4 to 30 ms, consistent with mitoBKca channel behavior.
Fig. 1. Outline of the mitochondrial patch-clamp experiments and comparison of the two types of recorded channel activities (Sek A, Kampa RP, et al., 2021).
Fig. 2. Biophysical properties of both mitoBKCa channels (channels with control and high activity) present in the inner mitochondrial membranes of human bronchial epithelial cells (Sek A, Kampa RP, et al., 2021).
ALT Treatment Suppressed CSE-induced Inflammation in Beas-2B and NHBE Cells
Remodeling and inflammation of the airways are hallmarks of COPD. Inflammation, apoptosis and oxidative stress are increased in COPD by smoking cigarettes smoke extract (CSE). Alantolactone (ALT), a sesquiterpene lactone from Inula helenium, has been found to disrupt these processes. Yet it remains unclear exactly how and why ALT plays such an important role in COPD pathology.
Deng et al. evaluated whether ALT reduced inflammation, apoptosis and oxidative stress in human bronchial epithelial Beas-2B cells and normal NHBE cells. They observed that ALT repressed CSE-induced inflammation, apoptosis and oxidative stress by activating the NF-B and Nrf2/HO-1 axises. CSE was infused into Beas-2B and NHBE cells to mimic in vitro pathology of COPD. In Beas-2B and NHBE cells, CSE was significantly higher in concentration (1, 2 and 5% vs. control) than it was in the control group for the following reasons: IL-1, TNF-, IL-6 and IFNY were increased by 24 h at Beas-2B and NHBE cells respectively (Fig. 3A and B). For further studies, they selected the concentration of CSE at 5%. They next tested the role of ALT in CSE-induced inflammation. Compared with the CSE exposure group, ELISA analysis of Beas-2B and NHBE cells confirmed a dose-dependent decrease of IL-1 β, TNF-a, IL-6 and IFN-Y secretion after 1, 5 and 10 μ M ALT exposure (Fig. 3C and D).
Fig. 3. ALT inhibited CSE induced - IL-1 β, TNF-α, IL-6 and IFN-γ production in human bronchial epithelial Beas-2B and normal bronchial epithelial cells NHBE (Dang, X., He, B., et al., 2020).
Our Bronchial Epithelial Cells are also available from Air-Liquid Interface pre-screened, Hypertension, and COPD/Asthma donors.
Creative Bioarray’s Bronchial Epithelial Cells (both normal and diseased) are isolated from the epithelial cells that line the airway of the bifurcation of the lungs and small sections of the bronchia just off of the bifurcation.
It is recommended to use SuperCult® Human Bronchial Epithelial Cell Growth Medium Kit (cat# CM-1351L) for the culturing of Human Bronchial Epithelial Cells.
Microbial infection and pathogenesis; airway inflammation and wound healing; asthma; pulmonary fibrosis, chronic obstructive pulmonary disease; chronic bronchitis; toxicology/other testing of pharmaceuticals.
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Average Rating: 5.0 | 1 Scientist has reviewed this product
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The quality and consistency of Creative Bioarray‘s bronchial epithelial cells have significantly enhanced our research results.
23 May 2023
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Description: The most abundant cell type in the bronchus is fibroblasts. They resemble a mesenchymal stem cell phenotype and their principle function is the production of type III collagen, elastin, and proteoglycans of the extracellular matrix. Bronchial fibroblasts play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial for effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts can result in abnormal tissue function. For example, the excess fibroblast proliferation and collagen secretion that occurs from bronchial subepithelial fibrosis can result in airway obstruction and bronchial hyper-responsiveness.
HBF are isolated from human bronchus tissue. HBF are cryopreserved at secondary culture (P1) and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBF are characterized by immunofluorescent method with antibody to fibronectin. HBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBF are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Description: The epithelium serves as the interface between the body and the external environment, covering all external surfaces and internal lumina. Epithelial cells, depending on their tissue of origin, perform various functions such as absorption, secretion, protection, transcellular transport, and sensation.
Human Tracheal Epithelial Cells (HTECs) from Creative Bioarray are isolated from the surface epithelium of healthy human tracheas, cryopreserved at passage 1, and shipped frozen. Each vial contains a minimum of 0.5 x 10^6 cells. These cells are tested and confirmed negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. They are guaranteed to achieve at least 12 population doublings under the conditions specified by Creative Bioarray.
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Creative Bioarray complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established.
Description: Creative Bioarray's normal Human Bronchial/Tracheal Epithelial Cells, when grown in Creative Bioarray's LIBro Medium, provide an ideal serum-free culture model for the accurate studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing. Creative Bioarray's HBTEC are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HBTEC are quality tested in LIBro B/T Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media.
Cell Features:
HBTEC are cryopreserved as primary cells. Cells are isolated from human bronchi/trachea and expanded once in culture vessels before being harvested for cryopreservation.
HSAEC are cryopreserved after primary cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation.
Creative Bioarray's Human Airway Epithelial Cells are not exposed to retinoic acid during isolation or expansion.
Human Airway Epithelial Cells are extensively tested for quality and optimal performance.
Creative Bioarray guarantees performance and quality.
Description: Human Primary Tracheal Fibroblasts are isolated from normal human tracheal tissue. Human Primary Tracheal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray' Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml.
Description: Epithelial cells were isolated from normal human bronchial epithelium obtained from autopsy of non-cancerous individuals.
