ML-2

Cat.No.: CSC-C0200

Species: Human

Source: acute myelomonocytic leukemia

Morphology: round, single cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0200

Description

Established from the peripheral blood of a 26-year-old man with acute myeloid leukemia (AML M4) at diagnosis of AML (following T-non-Hodgkin lymphoma and T-ALL) in 1978; carries t(6;11)(q27;q23) leading to MLL-MLLT4 (MLL-AF6) fusion gene; sister cell lines are ML-1 and ML-3

Species

Human

Source

acute myelomonocytic leukemia

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Culture Properties

suspension

Morphology

round, single cells in suspension

Karyotype

Human near tetraploid karyotype - 92(84-94)<4n>XX, -Y, -Y, -7, -9, -10, -10, +11, +12, +12, +13, +13, -15, -16, -17, -17, +18, +18, -20, -20, +4mar, der(1)t(1;?)(p21;?)x2, del(6)(q23)x2, der(6)t(6;11)(q27;?q23)x2, ?der(11)t(6;11)(q27;?q23)/del(11)(q23)x2,

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
mmunology: CD3 -, CD4 -, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +
Viruses: ELISA: rever

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

ML-2 cells are a well-known and extensively studied cell line that was established from the peripheral blood of a 26-year-old man in 1978. The blood sample was obtained at the time of diagnosis of acute myeloid leukemia (AML) of the M4 subtype. It is noteworthy that the patient had a medical history of T-cell non-Hodgkin lymphoma (T-NHL) and T-cell acute lymphoblastic leukemia (T-ALL) before the development of AML.

The establishment of the ML-2 cell line is significant because it carries a specific chromosomal translocation, t(6;11)(q27;q23), which results in the formation of the MLL-MLLT4 fusion gene, also known as the MLL-AF6 fusion gene. This fusion gene is a result of the rearrangement of the (MLL) gene on chromosome 11 and the myeloid/lymphoid or mixed-lineage leukemia translocated to the 4 (MLLT4) gene on chromosome 6.

ML-2 cells, along with their sister cell lines ML-1 and ML-3, have been instrumental in advancing our understanding of AML biology, particularly those associated with MLL gene rearrangements. Researchers have extensively utilized ML-2 cells to investigate the molecular mechanisms underlying leukemogenesis, disease progression, and response to various treatment modalities.

Lineage Infidelity of Human Myelogenous Leukemia Cell Lines

The organization and expression of the immunoglobulin heavy and light chain gene were analyzed in the human myeloblastic leukemic sublines, ML1, ML2, and ML3, and the human myeloid leukemic cell lines, HL-60, U937, THP1. One germline band of approximately 17 kb was detected in all the myeloid cell lines tested (Fig. 1). ML1, ML2, and ML3 cells showed an additional band of approximately 13.5 kb. Similarly, the joining (J_H) segment of the µ-gene, detected after hybridization of HindIII-digested DNA from the cell lines with a P-labeled J_H probe, resolved as a single 9-kb band in germline configuration in HL-60, U937, THP1, and K562 cells (Fig. 2A). However, the J_H segment was rearranged in ML1, ML2, and ML3 cells, as evidenced by a 9-kb germline band and a second band of 8 kb. Rearrangement of the J_H segment was also detected when the DNA samples were digested with EcoRI restriction endonuclease (Fig. 2B).

Fig. 1 C_µ-related sequences in DNA of human myeloid leukemic cell lines. (Lacrima K, et al., 2005)

Fig. 2 Configuration of J_H segment in human myeloid leukemic cell lines. (Lacrima K, et al., 2005)

What are circulating tumor markers?

Circulating tumor markers are used to estimate prognosis. determine the stage of cancer. detect cancer that remains after treatment (residual disease) or that has returned after treatment.

What genetic abnormality is present in ML-2 cells?

ML-2 cells carry the t(6;11)(q27;q23) chromosomal translocation, resulting in the formation of the MLL-MLLT4 (MLL-AF6) fusion gene. This fusion gene is a result of the rearrangement of the MLL gene on chromosome 11 and the MLLT4 gene on chromosome 6.

What is the significance of ML-2 cells in leukemia research?

ML-2 cells are an important research tool for studying AML associated with MLL gene rearrangements. They provide insights into the molecular mechanisms underlying leukemogenesis, disease progression, and treatment responses in this specific subtype of AML.

Are there any other cell lines related to ML-2 cells?

Yes, ML-2 cells have sister cell lines named ML-1 and ML-3. These cell lines are also derived from the same patient and share similar genetic characteristics, making them valuable for comparative studies and furthering our understanding of AML biology.

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Average Rating: 4.7 | 3 Scientist has reviewed this product

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Creative Bioarray has a wide range of cell types to choose from.

24 Apr 2023

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I've been using ML-2 cell products from Creative Bioarray for my research, and I'm impressed with the consistency and reliability of the cells.

24 Feb 2024

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High quality control

The quality control measures implemented by Creative Bioarray ensure that the cells exhibit the expected characteristics and maintain their genetic and phenotypic profile.

23 Nov 2023

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