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Human Bladder Epithelial Cells
Cat.No.: CSC-C4871L
Species: Human
Source: Bladder
Cell Type: Epithelial
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Never can cryopreserved cells be kept at -20 °C.
Human Bladder Epithelial Cells from Creative Bioarray are isolated from normal human bladder tissue. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials.
Our Primary bladder epithelial cells have been tested for the expression of the following biomarkers by immunocytochemistry staining: CK5 (+), CK7 (+), CK8 (+), CK18 (+), P63(nuclear) (+), Adherens junction (AJ) protein E-cadherin (+), Tight junction (TJ) protein - Zona occludens 1(ZO-1) (+) and α-smooth muscle Actin (-).
These cells proliferate very slowly and may take up to 10-14 days to fully recover from cryopreservation. After passaging, the cells may take about 7-10 days to reach 70-90% confluence, depending on the seeding density.
It is recommended to use SuperCult® Complete Human Epithelial Cell Medium (cat# CM-1098X) for the culturing of Human Bladder Epithelial Cells.
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
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Average Rating: 5.0 | 1 Scientist has reviewed this product
Great products and excellent support
1) High-quality product 2) Very well organised and excellent support from product delivery and experiment design practicalities.
13 Nov 2022
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Description: HBdSF from Creative Bioarray are isolated from human bladder tissue. HBdSF are cryopreserved at secondary culture (passage one) and are delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBdSF are characterized by immunofluorescent method with antibody to fibronectin. HBdSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdSF are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Description: HBdSMC from Creative Bioarray are isolated from human bladder tissue. HBdSMC are cryopreserved at secondary culture after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBdSMC are characterized by immunofluorescent method with antibodies to Α-smooth muscle actin and desmin. HBdSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdSMC are guaranteed to further expand for 15 population doublings in the condition provided by Creative Bioarray.
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Creative Bioarray complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established.
Description: Creative Bioarray's normal Human Bladder Epithelial Cells - Dome, when grown in LIUro D Complete culture medium, provide an ideal culture model for the study of bladder biology.
Description: Creative Bioarray's normal Human Bladder Epithelial Cells - Apex, when grown in LIUro A culture medium, provide an ideal culture model for the study of bladder biology.
Cell Features:
Human Bladder Epithelial Cells –Dome are cryopreserved as secondary cells, e.g. cells are isolated from the dome region of human bladder tissue and expanded in culture vessels twice before being harvested for cryopreservation.
Human Bladder Epithelial Cells –Apex are cryopreserved as secondary cells, e.g. cells are isolated from the apex/neck/trigone region of human bladder tissue and expanded in culture vessels twice before being harvested for cryopreservation.
Bladder cells are not exposed to phenol red or antimicrobials when cultured in Creative Bioarray medium.
Creative Bioarray guarantees performance and quality.
Description: Human Bladder Microvascular Endothelial Cells from Creative Bioarray are isolated from human bladder tissue. Human Bladder Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
