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Human Bladder Fibroblasts
Cat.No.: CSC-C4102X
Species: Human
Source: Bladder
Cell Type: Fibroblast
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1) The cells are cryopreserved at P2 and marketed as secondary cells to ensure the highest viability and proliferation efficiency. 2) Cells were identified: Fibronectin or Vimentin stained positive by immunofluorescence. 4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
It is recommended to use SuperCult® Complete Human Fibroblast Medium (cat# CM-1097X) for the culturing of Human Bladder Fibroblasts.
Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Our primary bladder fibroblasts are spindle-shaped and isolated from the urinary bladder of a donor. The cells can be used for the study or development of a potential diagnostic method for the early detection of bladder cancer cells, reconstruction studies, and advancement of cancer research.
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11 June 2023
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Description: HBdSF from Creative Bioarray are isolated from human bladder tissue. HBdSF are cryopreserved at secondary culture (passage one) and are delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBdSF are characterized by immunofluorescent method with antibody to fibronectin. HBdSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdSF are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Description: HBdSMC from Creative Bioarray are isolated from human bladder tissue. HBdSMC are cryopreserved at secondary culture after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBdSMC are characterized by immunofluorescent method with antibodies to Α-smooth muscle actin and desmin. HBdSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdSMC are guaranteed to further expand for 15 population doublings in the condition provided by Creative Bioarray.
Description: Creative Bioarray's normal Human Bladder Epithelial Cells - Dome, when grown in LIUro D Complete culture medium, provide an ideal culture model for the study of bladder biology.
Description: Creative Bioarray's normal Human Bladder Epithelial Cells - Apex, when grown in LIUro A culture medium, provide an ideal culture model for the study of bladder biology.
Cell Features:
Human Bladder Epithelial Cells –Dome are cryopreserved as secondary cells, e.g. cells are isolated from the dome region of human bladder tissue and expanded in culture vessels twice before being harvested for cryopreservation.
Human Bladder Epithelial Cells –Apex are cryopreserved as secondary cells, e.g. cells are isolated from the apex/neck/trigone region of human bladder tissue and expanded in culture vessels twice before being harvested for cryopreservation.
Bladder cells are not exposed to phenol red or antimicrobials when cultured in Creative Bioarray medium.
Creative Bioarray guarantees performance and quality.
Description: Human Bladder Epithelial Cells are isolated from normal human bladder tissue.
Description: Human Bladder Microvascular Endothelial Cells from Creative Bioarray are isolated from human bladder tissue. Human Bladder Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
