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Human Bladder Stromal Fibroblasts (HBdSF)
Cat.No.: CSC-7828W
Species: Human
Source: Bladder
Cell Type: Fibroblast; Stroma
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1) The cells are cryopreserved at passage one and are delivered frozen. 2) Cells are characterized by immunofluorescence with antibodies specific to fibronectin. 4) HBdSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
It is recommended to use SuperCult® Complete Human Fibroblast Medium (cat# CM-1097X) for the culturing of HBdSF in vitro.
Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Our primary bladder fibroblasts are spindle-shaped and isolated from the urinary bladder of a donor. The cells can be used for the study or development of a potential diagnostic method for the early detection of bladder cancer cells, reconstruction studies, and advancement of cancer research.
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Average Rating: 5.0 | 1 Scientist has reviewed this product
Convinent
This cell product is easy to culture and brings convenience to our experiments.
12 July 2022
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Description: HBdSMC from Creative Bioarray are isolated from human bladder tissue. HBdSMC are cryopreserved at secondary culture after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBdSMC are characterized by immunofluorescent method with antibodies to Α-smooth muscle actin and desmin. HBdSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdSMC are guaranteed to further expand for 15 population doublings in the condition provided by Creative Bioarray.
Description: Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. Creative Bioarray complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established.
Description: Creative Bioarray's normal Human Bladder Epithelial Cells - Dome, when grown in LIUro D Complete culture medium, provide an ideal culture model for the study of bladder biology.
Description: Creative Bioarray's normal Human Bladder Epithelial Cells - Apex, when grown in LIUro A culture medium, provide an ideal culture model for the study of bladder biology.
Cell Features:
Human Bladder Epithelial Cells –Dome are cryopreserved as secondary cells, e.g. cells are isolated from the dome region of human bladder tissue and expanded in culture vessels twice before being harvested for cryopreservation.
Human Bladder Epithelial Cells –Apex are cryopreserved as secondary cells, e.g. cells are isolated from the apex/neck/trigone region of human bladder tissue and expanded in culture vessels twice before being harvested for cryopreservation.
Bladder cells are not exposed to phenol red or antimicrobials when cultured in Creative Bioarray medium.
Creative Bioarray guarantees performance and quality.
Description: Human Bladder Epithelial Cells are isolated from normal human bladder tissue.
Description: Human Bladder Microvascular Endothelial Cells from Creative Bioarray are isolated from human bladder tissue. Human Bladder Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
