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Human Dermal Fibroblasts-fetal (HDF-f)
Cat.No.: CSC-7797W
Species: Human
Source: Dermis
Cell Type: Fibroblast
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Human dermal fibroblasts- fetal (HDF-f) are isolated from fetal dermal tissue and represent the most prevalent cell type within the loose connective tissue. These cells are typically located in close proximity to collagen fibers, to which they attach. Morphologically, they exhibit an elongated spindle shape and contain a cytoplasm rich in basophilic granules. Notably, their surface expresses high-affinity FcεRI receptors that bind free IgE, implicating them in type I hypersensitivity reactions. HDF-f are critical for the formation and development of fetal connective tissue, playing an indispensable role in embryonic development. HDF-f demonstrate a high proliferative capacity and vigorous metabolism, making them particularly amenable to in vitro culture and successive passaging.
The primary function of HDF-f is to synthesize and secrete extracellular matrix components such as collagen and elastin, which are crucial for maintaining skin firmness, elasticity, and moisture. Upon skin injury, these fibroblasts proliferate and migrate to the wound site, actively participating in the repair and regeneration process through the synthesis and secretion of matrix components. This capability is vital for preserving the integrity and function of fetal skin. Due to their developmental stage, HDF-f exhibit greater plasticity and possess a distinct gene expression profile compared to adult fibroblasts. Consequently, HDF-f hold significant potential for applications in tissue engineering and regenerative medicine, particularly given their enhanced capacity for regeneration and plasticity.
Fig. 1. 40X Inverted light microscopy of fetal dermal fibroblasts morphology (Goodarzi P, Falahzadeh K, et al., 2018).
hFDFs Exhibit In Vitro Multipotency and Displays an MSC Phenotype.
Developing allogeneic cell therapies that do not depend on large donor areas shows promise in addressing chronic and acute skin wound healing. Human fetal-derived dermal fibroblasts (hFDFs) are of particular interest due to their high division rates, low immunological rejection, and scarless healing ability. Esteban-Vives's team examined the potential of hFDFs as a source for cell banking.
Results indicated that hFDFs share markers and differentiation capabilities with bone marrow-derived mesenchymal stem cells (MSCs), demonstrating therapeutic potential. The hFDF cell populations demonstrated in vitro multipotency, similar to human MSCs. Proteoglycan staining post-induction confirmed osteogenic, adipogenic, and chondrogenic differentiation (Fig. 1A). hFDFs from six donors were cultured up to three passages, showing a decrease in CD105/CD90/CD73 expression from 57% to 15%, and an increase in CD34/CD45/CD14 expression from 0.1% to 2.6% by passage 3, maintaining CD79a/HLA-DR below 0.1% (Fig. 1B). Gene expression analysis from passages 4 to 7 corroborated these trends (Fig. 1C). Although hFDFs showed no significant growth rate differences compared to MSC-sorted hFDFs, their division rate was higher than BM-MSCs, suggesting suitability for cell banking (Fig. 1D).
Fig. 1. Detection of mesenchymal stem cell (MSC) population in human fetal dermal-derived fibroblasts (hFDFs) (Esteban-Vives R, Ziembicki J, et al., 2019).
Traction Forces Lower in Fetal Dermal Fibroblasts Than Adult
During adult wound healing, fibroblasts transition into myofibroblasts, generating forces that lead to scar formation. However, fetal wounds, which contain less transforming growth factor-ß1 and are softer, heal without scarring. Thus, Rachel's team explored whether the unique contractile behavior of fetal fibroblasts contributes to scarless repair.
Rachel's team tested whether physiological wound rigidity induces contractile forces in fetal dermal fibroblasts. Their previous work suggests that fetal fibroblasts have altered responses to ECM mechanical cues; therefore, they tested whether mechanosensing by adult and fetal dermal fibroblasts is differentially regulated by ECM rigidity. Fibroblasts were cultured on fibronectin-conjugated soft, hard, and rigid PAAs, corresponding to various wound healing stages, to measure contractile force generation (Fig. 2A). Both cell types showed similar force generation on soft and hard PAAs, but adult dermal fibroblasts exerted more force on rigid PAAs (Fig. 2B-C). This difference persisted after 18 hours (Fig. 2A-C). Using traction force microscopy, they once again found that adult dermal fibroblasts exerted larger traction forces than fetal dermal fibroblasts on rigid PAAs even though their cell sizes were the same (Fig. 2D-F). The results showed that traction forces were higher in adult dermal fibroblasts than in fetal ones after incubation.
Fig. 2. Fetal dermal fibroblasts exert less contractile force than adult dermal fibroblasts on rigid ECM (Rachel J., Jerrell, BS., et al., 2018).
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Description: Cells are isolated from adult abdominal dermal explants and provided either cryopreserved or in a variety of plated formats. Dermal Fibroblasts are available from donors with and without Type 2 diabetes. Donor information regarding gender, age, and BMI are provided. Bioarray provides at least 1x106 cells in each cryopreserved vial of dermal fibroblasts.
Description: Cells are isolated from adult abdominal dermal explants and provided either cryopreserved or in a variety of plated formats. Dermal Fibroblasts are available from donors with and without Type 2 diabetes.
Description: The melanocyte is a neural crest-derived cell that localizes in humans to several organs including the epidermis, eye, inner ear and leptomeninges. The failure of melanocytes to migrate to these locations explains the association of congenital white spotting of the skin (piebaldism) with heterochromia (the juxtaposition of different colors) in the iris as well as congenital deafness in Waardenburg syndrome. In the skin, melanocytes synthesize and transfer melanin pigments to surrounding keratinocytes, leading to skin pigmentation and protection against solar exposure. Recent progress in basic cell-culture technology, along with an improved understanding of culture requirements, has led to the success in culturing of this special cell type in pure population and the discovery of a novel melanocyte-specific gene, msg1, which encodes a nuclear protein and is associated with pigmentation.HEM from Bioarray Research Laboratories are isolated from neonate human epidermis. HEM are cryopreserved on passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HEM are characterized by immunofluorescent method with antibodies to fibronectin and NGF-receptor (p75). HEM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEM are guaranteed to further expand for 15 population doublings in the condition provided by Bioarray Research Laboratories.
Description: The melanocyte is a neural crest-derived cell that localizes in humans to several organs including the epidermis, eye, inner ear and leptomeninges. The failure of melanocytes to migrate to these locations explains the association of congenital white spotting of the skin (piebaldism) with heterochromia (the juxtaposition of different colors) in the iris as well as congenital deafness in Waardenburg syndrome. In the skin, melanocytes synthesize and transfer melanin pigments to surrounding keratinocytes, leading to skin pigmentation and protection against solar exposure. Recent progress in basic cell-culture technology, along with an improved understanding of culture requirements, has led to the success in culturing of this special cell type in pure population and the discovery of a novel melanocyte-specific gene, msg1, which encodes a nuclear protein and is associated with pigmentation.HEM from Bioarray Research Laboratories are isolated from neonate human epidermis. HEM are cryopreserved on passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HEM are characterized by immunofluorescent method with antibodies to fibronectin and NGF-receptor (p75). HEM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEM are guaranteed to further expand for 15 population doublings in the condition provided by Bioarray Research Laboratories.
Description: The melanocyte is a neural crest-derived cell that localizes in humans to several organs including the epidermis, eye, inner ear and leptomeninges. The failure of melanocytes to migrate to these locations explains the association of congenital white spotting of the skin (piebaldism) with heterochromia (the juxtaposition of different colors) in the iris as well as congenital deafness in Waardenburg syndrome. In the skin, melanocytes synthesize and transfer melanin pigments to surrounding keratinocytes, leading to skin pigmentation and protection against solar exposure. Recent progress in basic cell-culture technology, along with an improved understanding of culture requirements, has led to the success in culturing of this special cell type in pure population and the discovery of a novel melanocyte-specific gene, msg1, which encodes a nuclear protein and is associated with pigmentation.HEM-d from Bioarray Research Laboratories are isolated from neonate human epidermis. HEM-d are cryopreserved on passage one culture and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HEM-d are characterized by immunofluorescent method with antibodies to fibronectin and NGF-receptor (p75). HEM-d are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEM-d are guaranteed to further expand for 15 population doublings in the condition provided by Bioarray Research Laboratories.
Description: The melanocyte is a neural crest-derived cell that localizes in humans to several organs including the epidermis, eye, inner ear and leptomeninges. The failure of melanocytes to migrate to these locations explains the association of congenital white spotting of the skin (piebaldism) with heterochromia (the juxtaposition of different colors) in the iris as well as congenital deafness in Waardenburg syndrome. In the skin, melanocytes synthesize and transfer melanin pigments to surrounding keratinocytes, leading to skin pigmentation and protection against solar exposure. Recent progress in basic cell-culture technology, along with an improved understanding of culture requirements, has led to the success in culturing of this special cell type in pure population and the discovery of a novel melanocyte-specific gene, msg1, which encodes a nuclear protein and is associated with pigmentation.HEM from Bioarray Research Laboratories are isolated from adult human epidermis. HEM are cryopreserved on passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HEM are characterized by immunofluorescent method with antibodies to fibronectin and NGF-receptor (p75). HEM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEM are guaranteed to further expand for 15 population doublings in the condition provided by Bioarray Research Laboratories.
