- HCC-78
- HDLM-2
- DOHH-2
- L-540
- MX-1
- NALM-6
- NB-4
- CAL-51
- SNB-19
- KYSE-520
- MKN-45
- BA/F3
- MS-5
- HCEC-B4G12
- NK-92
- PA-TU-8988S
- MONO-MAC-1
- PA-TU-8902
- Human Microglia
- Human Hepatic Stellate Cells
- Human Skeletal Muscle Cells (DMD)
- Human Schwann Cells
- Human Oral Keratinocytes (HOK)
- Human Cardiomyocytes
- Human Small Intestinal Epithelial Cells
- Human Colonic Epithelial Cells
- Human Intestinal Fibroblasts
- Primary Human Large Intestine Microvascular Endothelial Cells
- Human Small Intestinal Microvascular Endothelial Cells
- Human Retinal Pigment Epithelial Cells
- Human Hepatocytes
- Cynomolgus Monkey Lung Microvascular Endothelial Cells
- Cynomolgus Monkey Vein Endothelial Cells
- C57BL/6 Mouse Primary Mammary Epithelial Cells
- C57BL/6 Mouse Vein Endothelial Cells
- Rat Primary Kidney Epithelial Cells
- Rat Gingival Epithelial Cells
- Rabbit Lung Endothelial Cells
Our Promise to You
Guaranteed product quality, expert customer support
ONLINE INQUIRY
Human Dermal Fibroblasts-adult (HDF-a)
Cat.No.: CSC-7798W
Species: Human
Source: Dermis
Morphology: Fibroblasts
Cell Type: Fibroblast
- Specification
- Background
- Scientific Data
- Q & A
- Customer Review
HDF-a are isolated from adult human skin. HDF-a are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HDF-a are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HDF-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HDF-a are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Never can primary cells be kept at -20 °C.
Human dermal fibroblasts-adult (HDF-a) is isolated from adult skin and is a key cell that constitutes the loose connective tissue of the skin. Typically displaying a spindle-shaped or stellate flattened structure, they possess prominent cellular protrusions and a large, distinct nucleus. These cells can be categorized into two subtypes: papillary fibroblasts and reticular fibroblasts. Papillary fibroblasts, adjacent to the epidermis and located in the upper dermis, exhibit higher proliferative capacity, play an active role in immune responses, and contribute to the maintenance of epidermal morphology. In contrast, reticular fibroblasts reside in the deeper dermis, surrounded by a thicker extracellular matrix, and primarily function to promote cytoskeletal remodeling and cell migration.
Given the crucial role of dermal fibroblasts in skin architecture and their amenability to in vitro culture, they are extensively utilized in cellular and molecular research. By combining HDFs with organic scaffolds or biocompatible extracellular matrices, it is possible to develop complex artificial tissues, organs, and even human analogs. Additionally, HDFs are employed in developing various skin repair products, including artificial dermis and skin grafts. As aging occurs, the efficacy of fibroblasts may diminish, potentially leading to skin aging characterized by increased laxity and wrinkle formation. Certain conditions, such as scleroderma and fibrotic diseases, are also associated with fibroblast dysfunction. Therefore, HDFs serve as crucial models for investigating the pathological mechanisms and potential therapeutic strategies for these diseases.
Fig. 1. Hman dermal fibroblast cell line staining with DDR-2 antibody and PI (Yun J, Park H, et al., 2010).
Inhibition Effects of Inotodiol on Oxidative Stress-Induced NOX5, MAPK and NF-ΚB in HDF Cells
Skin aging is caused by intrinsic factors such as changes in collagen production and extrinsic factors like pollution, UV radiation, and oxidative stress. Inotodiol, a lanostane triterpenoid compound from Inonotus obliquus, is known for its antiviral, anticancer, and anti-inflammatory properties; however, its ability to inhibit oxidative stress-induced human skin aging remains unclear.
Lee's team investigated the effect of inotodiol on oxidative stress in human dermal fibroblasts (HDF). Reactive oxygen species (ROS), especially H2O2, are key contributors to oxidative stress, damaging cells and leading to aging. NOX5 plays a key role in ROS production and is regulated by the MAPK pathway. They examined NOX5 expression in HDFs using WB, and the results indicated that NOX5 expression was upregulated after hydrogen peroxide stimulation, consistent with the level of NOX5 gene transcripts. HDF cells pretreated with inotodiol exhibited a reduction in hydrogen peroxide-induced NOX5 increase (Fig. 1a and b). Subsequently, the expression of MAPK and NF-κB was detected using WB, showing that MAPK and NF-κB expression was upregulated after hydrogen peroxide stimulation. HDF cells pretreated with inotodiol showed a reduction in hydrogen peroxide-induced MAPK and NF-κB activation increases (Fig. 2). To evaluate inotodiol's effects on oxidative stress-induced ERK1/2 activation in HDF cells, they used ELISA kits to measure phospho-ERK1/2. H2O2 treatment raised phospho-ERK1/2 levels, while inotodiol treatment reduced these levels. Thus, inotodiol inhibits oxidative stress-induced ERK1/2 and NF-κB expression in HDF cells.
Fig. 1. The effects of inotodiol on oxidative stress-induced NOX5 activation in HDF cells (Lee SH, Won GW, et al., 2022).
Fig. 2. The effects of inotodiol on oxidative stress-induced p-ERK1/2 and NF-κB-p65 activation in HDF cells (Lee SH, Won GW, et al., 2022).
Effects of HASC-derived EVs on the Migration of UVB-irradiated HDFs
Skin aging arises from intrinsic factors like natural aging and extrinsic factors such as UV radiation, which promotes photoaging through increased ROS production leading to collagen and elastin breakdown. Recently, extracellular vesicles (EVs) have gained attention for their potential in skin regeneration.
Choi's team explored the effects of EVs from human adipose-derived stem cells (HASCs) on UVB-induced photoaging of human dermal fibroblasts (HDFs). Normal HDFs were treated with serum-free medium and HASC-derived EVs. The EVs significantly increased type I collagen synthesis in a dose-dependent manner, with the highest synthesis at 5 × 108 particles/mL. UVB-irradiated HDFs also showed increased collagen synthesis with EVs, but no significant differences were observed between 1 and 5 × 108 particles/mL concentrations (Fig. 3). To study early cellular uptake, normal and UVB-irradiated HDFs were incubated with 1 × 108 particles/mL of fluorescent dye-labeled EVs for 3 hours. The EVs were internalized into the cytoplasm in both types of cells within this time, indicating high uptake efficiency. For migration studies, a scratch wound healing and transwell migration assay were performed. UVB-irradiated HDFs generally recovered slower than normal HDFs. However, wounds in UVB-irradiated HDFs treated with EVs closed faster than those without EVs or with BSA treatment (Fig. 4). In the transwell migration assay, normal HDFs migrated to the lower chamber with GM or EVs, while UVB-irradiated HDFs showed lower migration but increased migration with EVs. Also, EVs enhanced the proliferation of UVB-irradiated HDFs after 48 hours (Fig. 5). These findings suggest that HASC-derived EVs improve the migration and proliferation of UVB-irradiated HDFs.
Fig. 3. The cellular uptake of HASC-derived EVs in normal and UVB-irradiated HDFs (Choi JS, Cho WL, et al., 2019).
Fig. 4. The effects of HASC-derived EVs on the wound recovery of UVB-irradiated HDFs (Choi JS, Cho WL, et al., 2019).
Fig. 5. The effects of HASC-derived EVs on the (a) migration and (b) proliferation of UVB-irradiated HDFs (Choi JS, Cho WL, et al., 2019).
Ask a Question
Write your own review
Description: Cells are isolated from adult abdominal dermal explants and provided either cryopreserved or in a variety of plated formats. Dermal Fibroblasts are available from donors with and without Type 2 diabetes. Donor information regarding gender, age, and BMI are provided. Bioarray provides at least 1x106 cells in each cryopreserved vial of dermal fibroblasts.
Description: Cells are isolated from adult abdominal dermal explants and provided either cryopreserved or in a variety of plated formats. Dermal Fibroblasts are available from donors with and without Type 2 diabetes.
Description: The melanocyte is a neural crest-derived cell that localizes in humans to several organs including the epidermis, eye, inner ear and leptomeninges. The failure of melanocytes to migrate to these locations explains the association of congenital white spotting of the skin (piebaldism) with heterochromia (the juxtaposition of different colors) in the iris as well as congenital deafness in Waardenburg syndrome. In the skin, melanocytes synthesize and transfer melanin pigments to surrounding keratinocytes, leading to skin pigmentation and protection against solar exposure. Recent progress in basic cell-culture technology, along with an improved understanding of culture requirements, has led to the success in culturing of this special cell type in pure population and the discovery of a novel melanocyte-specific gene, msg1, which encodes a nuclear protein and is associated with pigmentation.HEM from Bioarray Research Laboratories are isolated from neonate human epidermis. HEM are cryopreserved on passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HEM are characterized by immunofluorescent method with antibodies to fibronectin and NGF-receptor (p75). HEM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEM are guaranteed to further expand for 15 population doublings in the condition provided by Bioarray Research Laboratories.
Description: The melanocyte is a neural crest-derived cell that localizes in humans to several organs including the epidermis, eye, inner ear and leptomeninges. The failure of melanocytes to migrate to these locations explains the association of congenital white spotting of the skin (piebaldism) with heterochromia (the juxtaposition of different colors) in the iris as well as congenital deafness in Waardenburg syndrome. In the skin, melanocytes synthesize and transfer melanin pigments to surrounding keratinocytes, leading to skin pigmentation and protection against solar exposure. Recent progress in basic cell-culture technology, along with an improved understanding of culture requirements, has led to the success in culturing of this special cell type in pure population and the discovery of a novel melanocyte-specific gene, msg1, which encodes a nuclear protein and is associated with pigmentation.HEM from Bioarray Research Laboratories are isolated from neonate human epidermis. HEM are cryopreserved on passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HEM are characterized by immunofluorescent method with antibodies to fibronectin and NGF-receptor (p75). HEM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEM are guaranteed to further expand for 15 population doublings in the condition provided by Bioarray Research Laboratories.
Description: The melanocyte is a neural crest-derived cell that localizes in humans to several organs including the epidermis, eye, inner ear and leptomeninges. The failure of melanocytes to migrate to these locations explains the association of congenital white spotting of the skin (piebaldism) with heterochromia (the juxtaposition of different colors) in the iris as well as congenital deafness in Waardenburg syndrome. In the skin, melanocytes synthesize and transfer melanin pigments to surrounding keratinocytes, leading to skin pigmentation and protection against solar exposure. Recent progress in basic cell-culture technology, along with an improved understanding of culture requirements, has led to the success in culturing of this special cell type in pure population and the discovery of a novel melanocyte-specific gene, msg1, which encodes a nuclear protein and is associated with pigmentation.HEM-d from Bioarray Research Laboratories are isolated from neonate human epidermis. HEM-d are cryopreserved on passage one culture and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HEM-d are characterized by immunofluorescent method with antibodies to fibronectin and NGF-receptor (p75). HEM-d are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEM-d are guaranteed to further expand for 15 population doublings in the condition provided by Bioarray Research Laboratories.
Description: The melanocyte is a neural crest-derived cell that localizes in humans to several organs including the epidermis, eye, inner ear and leptomeninges. The failure of melanocytes to migrate to these locations explains the association of congenital white spotting of the skin (piebaldism) with heterochromia (the juxtaposition of different colors) in the iris as well as congenital deafness in Waardenburg syndrome. In the skin, melanocytes synthesize and transfer melanin pigments to surrounding keratinocytes, leading to skin pigmentation and protection against solar exposure. Recent progress in basic cell-culture technology, along with an improved understanding of culture requirements, has led to the success in culturing of this special cell type in pure population and the discovery of a novel melanocyte-specific gene, msg1, which encodes a nuclear protein and is associated with pigmentation.HEM from Bioarray Research Laboratories are isolated from adult human epidermis. HEM are cryopreserved on passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HEM are characterized by immunofluorescent method with antibodies to fibronectin and NGF-receptor (p75). HEM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEM are guaranteed to further expand for 15 population doublings in the condition provided by Bioarray Research Laboratories.
