- HCC-78
- HDLM-2
- DOHH-2
- L-540
- MX-1
- NALM-6
- NB-4
- CAL-51
- SNB-19
- KYSE-520
- MKN-45
- BA/F3
- MS-5
- HCEC-B4G12
- NK-92
- PA-TU-8988S
- MONO-MAC-1
- PA-TU-8902
- Human Microglia
- Human Hepatic Stellate Cells
- Human Skeletal Muscle Cells (DMD)
- Human Schwann Cells
- Human Oral Keratinocytes (HOK)
- Human Cardiomyocytes
- Human Small Intestinal Epithelial Cells
- Human Colonic Epithelial Cells
- Human Intestinal Fibroblasts
- Primary Human Large Intestine Microvascular Endothelial Cells
- Human Small Intestinal Microvascular Endothelial Cells
- Human Retinal Pigment Epithelial Cells
- Human Hepatocytes
- Cynomolgus Monkey Lung Microvascular Endothelial Cells
- Cynomolgus Monkey Vein Endothelial Cells
- C57BL/6 Mouse Primary Mammary Epithelial Cells
- C57BL/6 Mouse Vein Endothelial Cells
- Rat Primary Kidney Epithelial Cells
- Rat Gingival Epithelial Cells
- Rabbit Lung Endothelial Cells
Our Promise to You
Guaranteed product quality, expert customer support
ONLINE INQUIRY
Human Osteoblasts-femural (HO-f)
Cat.No.: CSC-7744W
Species: Human
Source: Bone
Cell Type: Osteoblast
- Specification
- Q & A
- Customer Review
In order to test the ALP activity (osteogenic differentiation), cells were harvested using collagenase I and trypsin, centrifuged at 1500 rpm for 5 min, resuspended in 1 ml of cold PBS (4 °C) and counted. A volume corresponding to 300,000 cells was taken, centrifuged and suspended in 600 μl of assay buffer, and centrifuged at 13,200 rpm for 5 min at 4 °C. The supernatant was transferred to a new tube. Then 200 μl of the supernatant was distributed in four wells of a 96-well plate (50 μl/well). One of these wells served to determine the background through the simultaneous addition of 80 μl p-nitrophenyl phosphate (pNPP) 1 mM and 20 μl of stop solution. For the three remaining wells, 80 μl of pNPP was added. After 60 min, 20 μl of stop solution was added to the wells and the plate was read in a spectrophotometer to measure the optical density at 405 nm. The quantity of p-nitrophenol (pNP) in each well was determined using a standard curve established using pNPP and purified ALP enzyme. Then 350 μl of the remaining supernatant was incubated with 750 nM of Hoechst 33342 for 30 min and distributed in triplicate in an opaque 96-well plate to read the fluorescence at 365 nm excitation.
Ask a Question
Average Rating: 5.0 | 1 Scientist has reviewed this product
It gives outstanding results
The cell gave excellent results.
09 June 2022
Ease of use
After sales services
Value for money
Write your own review
Description: Human Calvarial Osteoblasts (HCO) from Creative Bioarray are isolated from human calvariae. HCO are cryopreserved at primary cultures and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCO are characterized by the cytochemically detection of AP and mineral deposition. HCO are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCO are guaranteed for 15 population doublings at the conditions provided by Creative Bioarray.
Description: Human osteoblasts cells are derived from femoral trabecular bone tissue from the knee or hip joint region. They stain positive for alkaline phosphatase and can be mineralized.
