Human Osteoblast Cells

Cat.No.: CSC-7860W

Source: Bone

Cell Type: Osteoblast

  • Specification
  • Q & A
  • Customer Review
Cat.No.
CSC-7860W
Description
Human osteoblasts cells are derived from femoral trabecular bone tissue from the knee or hip joint region. They stain positive for alkaline phosphatase and can be mineralized.
Source
Bone
Cell Type
Osteoblast
Disease
Normal
Storage and Shipping
Store at -196 °C (LN2) upon receipt until ready to culture. 10% DMSO
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
Where are Human Osteoblasts (HOB) isolated from?

Creative Bioarray’s Human Osteoblasts are isolated by explant, and the tissue is from the spongy section of the bone head of the ribs, femur, or tibia.

Are the osteoblast cells contact inhibited?

No, our osteoblast cells are not contact inhibited and tend to grow on top of each other after becoming confluent.

What is the approximate doubling time of osteoblasts?

The average doubling time of our osteoblasts is 35-40 hours in earlier passages. If in older (later passage) cultures, it can be much longer, and there might be donor-to-donor variations as well.

Why does Creative Bioarray recommend two different concentrations of the β-Glycerophosphate in the differentiation protocol?

We have found some osteoblast cell strains to be more sensitive to β-Glycerophosphate than others. Therefore, we recommend trying two cited concentration to determine the optimal concentration for each specific lot.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

So fast and helpful

The Creative Bioarray Team has been extremely helpful and patient in assisting me in placing orders for cells and recommending appropriate media and protocols. Any orders I have placed have arrived faster than expected and have grown without issue.

12 May 2022


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