Human Calvarial Osteoblasts (HCO)

Creative Bioarray’s osteoblasts generally appear fibroblastic. They are cuboidal in lower densities and become spindle shaped as they become confluent.

It is not easy to give an exact number for the purity of osteoblasts as the tests used are all functional assays: alkaline phosphatase production and bone mineralization, which unless you have a synchronized culture, not all the cells are doing the same thing at the same time. Based on the cell morphology and other parameters, we consider the purity of the osteoblast culture to be approximately 95%.

It is recommended to use SuperCult® Osteoblast Growth Medium Kit (cat# CM-1149X) for the culturing of primary human osteoblasts.

You can either use chemical elution or digital image quantification. Based on Alizarin Red's fluorescence properties, fluorescence spectroscopy might work, as well. No matter which tool you are going to use, make sure that your pH is well calibrated. Otherwise your dye will change colour between yellow, red and purple.