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Chronic Myeloid Leukemia Bone Marrow CD34+ Cells(Chronic Phase)
Cat.No.: CSC-C4545X
Species: Human
Source: Bone Marrow
Cell Type: CD34+
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CD34+ cells make up about 2% of the population in bone marrow mononuclear cells.
We recommend the following thawing procedure: Rapidly thaw the cryovial in a 37°C water bath (1-2 min). Remove the vial before all the ice is thawed. Decontaminate the cryovial with 70% ethanol and move it to a biosafety cabinet and keep on ice. While on ice, transfer contents of the vial to a 15mL conical tube. Rinse the cryovial with 1mL of thawing media and slowly transfer to the cells to the conical tube. Slowly pipet 10X the vial volume of thawing media into the conical tube. Take a sample for counting and viability assessment. Centrifuge the cells at 4°C at 300 x g for 5 minutes to pellet cells. Aspirate the supernatant carefully, leaving a few mLs behind. Re-suspend cell pellet in the assay media of your choice.
When resting, they are about 8-10 microns.
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Description: Human bone marrow stromal cells or mesenchymal stem cells (MSCs) are derived from bone marrow mononuclear cells. MSCs are isolated from a single donor. MSCs are one of the most investigated cells for therapeutic applications. These cells enable researchers to investigate stem cell properties, regenerative medicine, and tissue engineering. In addition, they may be used for developmental studies.
Development period: Postnatal
Description: Human bone marrow plasma is the liquid component of blood. Bone marrow plasma consists of dissolved proteins, glucose, clotting agents, minerals, hormones, and carbon dioxide. It makes up approximately 55% of the whole bone marrow suspension. This whole bone marrow plasma is isolated from a single donor. The plasma is separated out from the whole bone marrow (sent separately) and immediately frozen at -80°C in order to preserve its contents. Whole bone marrow plasma enables researchers to investigate specific components of bone marrow at the molecular level.
Development period: Postnatal
Description: Waldenstrom's Macroglobunemia-Bone Marrow-Mononuclear Cells are density separated by Ficoll-Paque to enrich mononuclear cells from the bone marrow. Waldenstrom's Macroglobunemia-Bone Marrow-Mononuclear Cells are available in untreated and relapsed/refractory stages.
Description: Bone Marrow is obtained from adult Myelodysplastic Syndrome patients. Bone Marrow-Mononuclear Cells are isolated by diluting the bone marrow with phosphate-buffered saline and using gradient separation techniques. After centrifugation,the Mononuclear Cells layer is collected. Mononuclear Cells can be processed further to isolate cell subpopulations. Myelodysplastic Syndrome-Mononuclear Cells are available in the newly diagnosed and relapsed/refractory stages.
Description: Bone Marrow is obtained from adult Myelodysplastic Syndrome patients. Bone Marrow-Mononuclear Cells are isolated by diluting the bone marrow with phosphate-buffered saline and using gradient separation techniques. After centrifugation,the Mononuclear Cells layer is collected. Mononuclear Cells can be processed further to isolate cell subpopulations. Myelodysplastic Syndrome-Mononuclear Cells are available in the newly diagnosed and relapsed/refractory stages.
Description: Multiple Myeloma-Bone Marrow Plasma is obtained by centrifuging the Multiple Myeloma-bone marrow. The plasma has a diminished number of cells and platelets. Multiple Myeloma-Bone Marrow plasma is available in both the newly diagnosed and relapsed/refractory stages.
