Acute Myeloid Leukemia Bone Marrow-CD34+ Cells (Newly Diagnosed)

Cat.No.: CSC-C4585X

Species: Human

Source: Bone Marrow

Cell Type: CD34+

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Cat.No.
CSC-C4585X
Description
Acute Myeloid Leukemia-Bone Marrow-CD34+ cells are selected from adult Acute Myeloid Leukemia-Bone Marrow-Mononuclear Cells. The Bone Marrow-CD34+ stem/progenitor cells are positively isolated using a direct immunomagnetic CD34 MicroBead labeling system. Acute Myeloid LeukemiaCD34+ cells are available in the newly diagnosed and relapsed/refractory stages.
Species
Human
Source
Bone Marrow
Cell Type
CD34+
Disease
Diseased
Storage and Shipping
Store in liquid nitrogen and ship in dry ice.
Citation Guidance
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Are they suspension cells or do they partially attach?

It actually depends on the culture conditions whether the cells remain in suspension or attach to the surface.

Can I expand the CD34+ progenitors without differentiating them?

No, the cells will proliferate and differentiate simultaneously upon being placed in culture. Varying the cytokine cocktail can influence the differentiation and proliferation of the cells.

What cell density should we culture at for 6-well plates? 24-well plates? 96-well plates?

Each immune cell type has its own recommended, specific cell densities for culture. Please refer to the literature for cell densities that are most appropriate for your research application.

What happens if I grow CD34+ or CD133+ progenitors in hematopoietic progenitor cell expansion medium without cytokines?

The cells will die. Hematopoietic progenitor cell expansion medium always must be supplemented with an appropriate cocktail of cytokines.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

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11 May 2023


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