Porcine Small Intestinal Fibroblasts

Cat.No.: CSC-C4901L

Species: Porcine

Source: Intestine

Cell Type: Fibroblast

  • Specification
  • Q & A
  • Customer Review
Cat.No.
CSC-C4901L
Description
Porcine Intestinal Fibroblasts from Creative Bioarray are isolated from intestinal tissue of porcine. Porcine Intestinal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution and incubated in Creative Bioarray Complete Growth Medium. The cells are shipped at passage 2. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen.
Species
Porcine
Source
Intestine
Cell Type
Fibroblast
Disease
Normal
Quality Control
Porcine Primary Intestinal Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Porcine Primary Intestinal Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
How many days to change the medium?

Under normal conditions, the culture medium is reddish. If the cells are maintained at pH 6.5-6.6, the medium turns yellow, indicating that the metabolites in the culture medium have accumulated to a certain amount, the cells will fall off and die, and it is necessary to replace the fresh culture medium. Generally, the culture medium should be changed once every 1~2 days when the cells are growing vigorously, and once every 3~4 days when the cells are growing slowly. For resuscitated cells, it is recommended to change the culture medium every other day.

Ask a Question

Average Rating: 4.0    |    1 Scientist has reviewed this product

Delighted

I have been using Creative Bioarray for years and I have always been delighted with their products and services.

12 Aug 2023


Ease of use

After sales services

Value for money


Write your own review

For research use only. Not for any other purpose.