Porcine Artery Endothelial Cells

Cat.No.: CSC-C4205X

Species: Porcine

Source: Artery

Cell Type: Endothelial

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Cat.No.
CSC-C4205X
Description
Porcine Artery Endothelial Cells are derived from healthly porcinine artery. Porcine Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Creative Bioarray's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.
Porcine Artery Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
Species
Porcine
Source
Artery
Recommended Medium
Porcine Endothelial Cell Medium
Cell Type
Endothelial
Disease
Normal
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.

Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
Why does the culture medium become dark red in color and increasingly alkaline in pH when stored in a 4 C refrigerator?

When the medium is stored in a 4°C refrigerator, the CO2 in the medium will gradually escape, causing the medium to become more alkaline, and the color of the acid-base indicator (usually phenol red) in the medium will become more dark red as the alkalinity increases. The result of alkaline medium will cause cell growth stagnation or death. If the medium is alkaline, the pH can be adjusted by introducing sterile filtered CO2.

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