Porcine Brain Vascular Smooth Muscle Cells

Cat.No.: CSC-C4909L

Species: Porcine

Source: Brain

Cell Type: Smooth Muscle

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Cat.No.
CSC-C4909L
Description
Porcine Brain Vascular Smooth Muscle Cells from Creative Bioarray are isolated from brain tissue of Porcine. Porcine Brain Vascular Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution and incubated in Creative Bioarray Complete Growth Medium. The cells are shipped at passage 2. Each vial contains at least 0.5x10^6 cells. The cells are characterized by immunofluorescent method with antibodies to α-smooth muscle actin. Porcine Brain Vascular Smooth Muscle Cells from Creative Bioarray are negative for bacteria, yeast, fungi, and mycoplasma. Cells are guaranteed to further expand for 3 passages following the instructions provided by Creative Bioarray Repeated freezing and thawing of cells is not recommended.
Species
Porcine
Source
Brain
Cell Type
Smooth Muscle
Disease
Normal
Quality Control
Porcine Brain Vascular Smooth Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
How do I count live cells with Teparan?

Dilute the cell suspension to 200-2000 cells/mL with serum-free medium, and add 0.1 mL of 0.4% Teparan solution to 0.1 mL of cell suspension. Mix gently and count the cells with a hematocrit plate. Live cells reject Teparan, so cells stained blue are dead cells. Note that counting should be completed within 10 min of adding Teparan. If you have a cell counter, you can use the counter directly.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Much thanks

Thanks to Creative Bioarray's staff for their kind help, making my experiment successful.

12 Aug 2023


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