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Porcine Aortic Smooth Muscle Cells
Cat.No.: CSC-C4906L
Species: Pig
Source: Aorta
Cell Type: Smooth Muscle Cell
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Never can cryopreserved cells be kept at -20 °C.
In general the cell growth to complete confluence should be passaged, all cell growth has a requirement should not grow too dense (that is, often referred to as old), but there is contact inhibition of the cell, before confluence must be passaged, such cells are generally in the density of 70-80% on the passaging, otherwise it will cause cell differentiation.
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Average Rating: 5.0 | 1 Scientist has reviewed this product
Consistent and reliable
The product I found has certainly exceeded my expectations. It has remained consistent and reliable through many long-term usages.
12 Aug 2023
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Description: Porcine Vein Endothelial Cells from Creative Bioarray are isolated from inferior vena cava tissue of porcine. Porcine Vein Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Description: Porcine Pulmonary Vein Endothelial Cells from Creative Bioarray are isolated from pulmonary vein of porcine. Porcine Pulmonary Vein Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Description: Porcine Pulmonary Artery Endothelial Cells from Creative Bioarray are isolated from pulmonary artery of porcine. Porcine Pulmonary Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Description: Porcine Brain Microvascular Endothelial Cells from Creative Bioarray are isolated from brain tissue of porcine. Porcine Brain Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
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Porcine Artery Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.
