Porcine Uterine Microvascular Endothelial Cells

Cat.No.: CSC-C8661W

Species: Porcine

Source: Uterus

Cell Type: Endothelial

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Cat.No.
CSC-C8661W
Description
Porcine Uterine Microvascular Endothelial Cells from Creative Bioarray are isolated from uterine tissue of porcine. Porcine Uterine Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Species
Porcine
Source
Uterus
Cell Type
Endothelial
Disease
Normal
Quality Control
Porcine Uterine Microvascular Endothelial Cells are tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Porcine Uterine Microvascular Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
Do serum-free cultures use the same amount of antibiotics as serous cultures?

When adding antibiotics to serum-free media, reduce the concentration used in serum-bearing media by at least 50%. Serum proteins bind and inactivate some antibiotics. Under serum-free culture conditions, antibiotics are not inactivated and may reach toxic levels for cells.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

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We have been working with cells for many years and trust Creative Bioarray's products.

13 Jan 2023


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