Porcine Dermal Lymphatic Endothelial Cells

Cat.No.: CSC-C8636W

Species: Porcine

Source: Dermis

Cell Type: Endothelial

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Cat.No.
CSC-C8636W
Description
Porcine Dermal Lymphatic Endothelial Cells from Creative Bioarray are isolated from skin tissue of porcine. Porcine Dermal Lymphatic Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Species
Porcine
Source
Dermis
Cell Type
Endothelial
Disease
Normal
Quality Control
Porcine Dermal Lymphatic Endothelial Cells are tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Porcine Dermal Lymphatic Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What is the key to lymphatic vessel endothelial cell isolation and culture?

The ability of the thoracic duct tissue block to adhere to the wall is critical for endothelial cell culture. Therefore, it is important to be gentle when adding the culture medium so as not to float the tissue block.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Very nice

After we use your cells, the experiment yielded good results.

09 Jun 2023


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