Porcine Coronary Artery Endothelial Cells

Cat.No.: CSC-C8634W

Species: Porcine

Source: Artery

Cell Type: Endothelial

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Cat.No.
CSC-C8634W
Description
Porcine Coronary Artery Endothelial Cells from Creative Bioarray are isolated from coronary artery of porcine. Porcine Coronary Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Species
Porcine
Source
Artery
Cell Type
Endothelial
Disease
Normal
Quality Control
Porcine Coronary Artery Endothelial Cells are tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Porcine Coronary Artery Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
How do I freeze the cells?

The cells are collected by centrifuge, the supernatant is thoroughly removed from the centrifuge tube, and the appropriate amount of serum-free cell lyophilized solution is added to the tube to make a cell lyophilized suspension, which is placed in a -80°C refrigerator for long-term freezing or transferred to liquid nitrogen.

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Average Rating: 4.0    |    1 Scientist has reviewed this product

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10 May 2023


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