Human Hypertension Artery Smooth Muscle Cells

Cat.No.: CSC-C5005J

Species: Human

Source: Artery

Cell Type: Smooth Muscle

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Cat.No.
CSC-C5005J
Description
Human hypertension artery smooth muscle cells are isolated from the carotid artery of human donor that has been diagnosed with hypertension. Human hypertension artery smooth muscle cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in complete growth medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from the culture flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen.
Species
Human
Source
Artery
Cell Type
Smooth Muscle
Disease
Diseased
Quality Control
Human hypertension artery smooth muscle cells are negative for bacteria, yeast, fungi and mycoplasma.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What are the reasons why cells start to die gradually after two generations of passaging?

It is likely that the culture system is not suitable for the cells (the recommended culture system is not used); or the digestion is excessive, which has a serious effect on the cells, or the passaging ratio is inappropriate (cells with density-dependent properties are passaged too sparsely, or cells with faster growth are passaged more densely, and the cells grow in severe stacks).

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Good quality control

The quality control of the cell products is very good, as demonstrated by the good reproducibility and the ease of handling steps.

13 Feb 2021


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