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Human Hypertension Artery Endothelial Cells
Cat.No.: CSC-C4984J
Species: Human
Source: Artery
Cell Type: Endothelial
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Never can cryopreserved cells be kept at -20 °C.
If it is suspension cells, collect the cell supernatant and centrifuge it slowly (500-600 rpm/min, 5-6 min) and replace it with a new culture flask. If it is wall-adherent cells, wash the cells with PBS for 2-3 times, when washing, gently tap the flask to dislodge the fragments and particles that are not firmly adhered to the wall, and then discard the PBS, digest the cells by adding low concentration trypsin such as 0.05% trypsin for 1 min or so, let the particles and fragments in the cell gap fall off, remove the low concentration trypsin, and then digest the cells normally. around, let the particles and fragments in the cell gap fall off, remove the low concentration of trypsin, then digest the cells normally, centrifuge the collected cell suspension slowly (500-600 rpm/min, 5-6 min) and replace it with a new culture flask, and you can try to increase the concentration of serum appropriately for culture.
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Average Rating: 5.0 | 1 Scientist has reviewed this product
Good experimental results
We have had good experimental results with U87 / WT for brain cell research.
15 Jan 2021
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