Human Hypertension Aortic Smooth Muscle Cells

Cat.No.: CSC-C5004J

Species: Human

Source: Aorta

Cell Type: Smooth Muscle

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Cat.No.

CSC-C5004J

Description

Human hypertension aortic smooth muscle cells are isolated from the aorta of human donor that has been diagnosed with hypertension. Human hypertension aortic smooth muscle cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in complete growth medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from the culture flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. The cells are characterized by immunofluorescent method with antibodies to α-smooth muscle actin.

Species

Human

Source

Aorta

Recommended Medium

SuperCult® Human Smooth Muscle Cells Growth Medium Kit

Cell Type

Smooth Muscle

Disease

Diseased

Quality Control

Human hypertension aortic smooth muscle cells are negative for bacteria, yeast, fungi and mycoplasma.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Is it normal to have vacuoles in the cells?

It is normal for some cells to have some vacuoles (e.g. HepG2, Ishikawa and some drug-resistant strains). If only a small number of cells have very few vacuoles, it is likely that the cell state is not good, and the cell state can be adjusted by adjusting the serum concentration, controlling digestion, and controlling the ratio and time of passaging, etc.. If most of the cells have vacuoles, and the number of vacuoles in a single cell is relatively large, it is likely to be caused by the aging of the cell, and it is necessary to change the cell with an earlier generation.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

A lot of guidance

I used NGP cells in my experiments and the technicians gave a lot of guidance on the media and reagents.

19 Feb 2021

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For research use only. Not for any other purpose.