eNOS KO Mouse Colonic Smooth Muscle Cells

Cat.No.: CSC-C8490W

Species: Mouse

Source: Intestine

Cell Type: Smooth Muscle

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Cat.No.
CSC-C8490W
Description
eNOS KO Mouse Colonic Smooth Muscle Cells from Creative Bioarray are isolated from colonic tissue of pathogen-free laboratory mice. eNOS Knockout Mouse Colonic Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. The cells are characterized by immunofluorescent method with antibodies to α-smooth muscle actin. eNOS Knockout Mouse Colonic Smooth Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray (Cells may be expanded for 2 or 3 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray). Repeated freezing and thawing of cells is not recommended.
Species
Mouse
Source
Intestine
Cell Type
Smooth Muscle
Disease
Normal
Quality Control
Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray (Cells may be expanded for 2 or 3 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray). Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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