TLR4 KO Mouse Lymphatic Endothelial Cells

Cat.No.: CSC-C8742W

Species: Mouse

Source: Lymph

Cell Type: Endothelial

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Cat.No.
CSC-C8742W
Description
Toll-Like Receptor 4 Knockout (TLR4 KO) Mouse Lymphatic Endothelial Cells from Creative Bioarrayare isolated from lymph node tissue of pathogen-free laboratory mice. Toll-Like Receptor 4 Knockout (TLR4 KO) Mouse Lymphatic Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with LYVE1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
Species
Mouse
Source
Lymph
Cell Type
Endothelial
Disease
Normal
Quality Control
Toll-Like Receptor 4 Knockout (TLR4 KO) Mouse Lymphatic Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R&D System), CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) or LYVE-1 (Catalog No. ab9498, Abcam) by immunofluorescence staining or FACS. Toll-Like Receptor 4 Knockout (TLR4 KO) Mouse Lymphatic Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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